Products, services, reviews and techniques used in the identification and validation of the disease-causing target genes and proteins.
The StrataClone Mammalian Expression Vectors were designed for high-level expression of epitope-tagged proteins in mammalian cells. Use of StrataClone technology for the cloning steps allows rapid cloning of blunt-ended PCR products, produced using high-fidelity PCR enzymes, into the epitope tagging/expression vectors.
Our Vitality hrGFP (humanized, Renilla reniformis green fluorescent protein) products are optimal for mammalian expression protein studies that examine gene expression, transfection efficiency, and protein localization. Since hrGFP is derived from a different organism than EGFP, the hrGFP antibody does not cross react with the Aequorea victoria GFP variant-derived EGFP.
Our Mycoplasma Plus PCR Primer Set detects infection by all forms of Mycoplasma with a robust single-band PCR product (triplet for Acholeplasma). This kit also includes controls to verify PCR amplification and Mycoplasma genomic DNA. The primer design permits higher annealing temperatures, reducing spurious bands from PCR contaminants.
The MycoSensor PCR & QPCR Assay Kits detect Mycoplasma infection of cell cultures by real-time quantitative PCR (qPCR) or PCR in less than two hours.
Our SideStep lysis and Stabilization buffer ensures accurate gene expression studies by eliminating sample loss and degradation. Our proprietary buffer lyses cells and immediately stabilizes the released nucleic acid for months. Following the 10-minute room temperature lysis, you can go directly from cells to QPCR amplification without stopping to purify.
pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives.
The GeneMorph II Random Mutagenesis Kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis. Other methods or commercially available kits that rely on Taq DNA polymerase for random mutagenesis have shown biases towards mutating A’s and T’s more frequently than G’s and C’s, which undoubtedly skews representation of random mutant libraries.
The GeneJammer Transfection Reagent is a proprietary formulation of novel polyamines ideal for transient or stable transfections in a wide variety of cell types. This transfection reagent is effective in both serum-containing medium and serum-free medium, offering high efficiencies with minimal cytotoxicity.
Hygromycin B is an antibiotic that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.
The LipoTAXI mammalian transfection reagent contains a unique, low-toxicity lipid formulation that has been tested with over 30 cell lines and has been shown to achieve high efficiency transfections with a wide variety of cells. Compared to CaPO4/DEAE-mediated transfection, it provides a 10-fold increase in transient transfection efficiency in many cell lines. This transfection reagent may be used with both adherent cells and…
