Drug discovery > Target Discovery Products & Reviews

The basic BL21 strain does not contain the T7 RNA polymerase gene and can be used with non-T7 RNA polymerase protein expression systems. 

Replicating eukaryotic DNA in prokaryotic cells can be problematic. Particular eukaryotic genes may contain inverted repeats or secondary structures, such as Z-DNA, that can be rearranged or deleted by E. coli DNA repair systems. The SURE competent cells are deficient in the E. coli genes involved in the rearrangement and deletion of DNA, thus improving cloning efficiencies of DNA containing irregular structures in prokaryoti…

These competent cells offer the highest transformation efficiencies available, enabling dramatically improved transformation efficiencies when cloning large plasmids and ligated DNA.

This collection of competent cells provides customers with classic E. coli strains that have been engineered to become competent for use in cloning experiments.

This collection of competent cells provides customers with classic E. coli strains that have been engineered to become competent for use in cloning experiments.

 Agilent offers the SCS110 and JM110 strains, which lack both dam and dcm activity. DNA propagated in these strains can be digested by methylation-sensitive enzymes such as Xba I, Cla I and EcoR II. SCS110 is an endA– derivative of the JM110 strain.

This collection of competent cells provides customers with classic E. coli strains that have been engineered to become competent for use in cloning experiments.

The pCMV-Script PCR cloning kit is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives. In the ligation reaction, PCR products are incubated with the predigested pCMV-Script mammalian expression vector, the restriction enzyme Srf I, and T4 DNA ligase.  Cl…

BL21-Gold competent cells feature the high transformation efficiency phenotype (Hte) and have the gene encoding endonuclease I (endA) inactivated. 

The XL1-Blue strains are an all-prupose line of competent cells that are ideal for routine cloning needs.