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Products, services, reviews and techniques used in the identification and validation of the disease-causing target genes and proteins.

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Dual-Light® Combined Reporter Gene Assay System for Detection of Luciferase and beta-Galactosidase

Thermo Fisher Scientific

Dual-Light® luminescent reporter gene assay is designed for the rapid and sensitive detection of firefly luciferase and b-galactosidase in a single extract aliquot for gene expression assays. Detection of firefly luciferase and b-galactosidase in the same sample simplifies normalization of transfection efficiency Wide dynamic range of b-galactosidase and luciferase assays enables accurate measurement of both enzymes from…

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IPTG

Thermo Fisher Scientific

IPTG (Isopropyl-beta-D-thiogalactoside) is an inducer of the lac operon and is frequently used in conjunction with X-GAL to determine the lac phenotype of recombinant plasmids or phage. IPTG is also used to induce the expression of cloned genes that are under control of the lac operon.

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X-GAL

Thermo Fisher Scientific

X-GAL (5-Bromo-4-Chloro-3 Indolyl-Beta-D-thiogalactopyranoside) is a chromogenic substrate for Beta-galactosidase that turns blue upon hydrolysis and is regularly used for the blue/white color selection assay. Bacteria with a lac+ phenotype grow as blue colonies on plates containing X-GAL.

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