Antibodies and Conjugates
DAB (3,3-diaminobenzidine tetrahydrochloride), is used as a chromogen substrate in immunoblotting.DAB produces a brown colour during the enzymatic degradation of H2SO4 by horseradish peroxidase. Each tablet contains 10 mg of DAB and dissolves in distilled water in less than 5 minutes. Stability life time is 18 months at 4°C.
Fast Red is used as a chromogen substrate in immunoblotting and immunohistochemistry using alkaline phosphatase antibody conjugates. Each tablet contains 0.5 mg naphthol phosphate, 2 mg FAST RED chromogen and 0.4 mg levamisole, an inhibitor of endogenous enzyme activity. Stability life time is 18 months at 4°C.
The pNPP (p-NitroPhenyl Phosphate) liquid, ready-to-use substrate is a highly active and stable formulation utilized for measuring AP- (Alkaline Phosphatase) probe activity. The solution is a solvent free buffer solution containing stabilized pNPP using sodium azide as preservative. In kinetic assays p-nitrophenol appears yellow and is measured at 405 /650 nm. If stopped reactions are preferred, add equal amounts of 1 .0 M s…
The liquid BCIP/NBT is the most commonly used substrate for colorimetric determination of Alkaline Phosphatase (AP) activity in membrane tests. We offer an aqueous buffered solution containing NBT (NitroBlue Tetrazolium) and BCIP (Bromo-Chloro-Indolyl Phosphate) forming a blue/purple precipitate at the positive sites. The colour develops when AP catalyses the dephosphorylation of BCIP. The intense blue/purple precipitate is…
TMB (3,3´,5,5´- tetramethylbenzidine) is a substrate for horseradish peroxidase (HRP). Our mono-component, liquid TMB solutions combine TMB, substrate buffer and hydrogen peroxide for a safe, ready-to-use, one step, chromogen system. The products do not contain harmful solvents. Recommended for ELISA procedures, liquid TMB yields a soluble blue end product read at 655 nm. The TMB reaction may be stopped with 0.2 M H2SO4 resu…
This new type of TMB with a red indicator combines all the important qualities of TMB ONE, eliminating possible mistakes during pipetting. Further the red colour may be useful in automatic analyzers giving the warning "Reagent low" when it is time for filling up the substrate.The red indicator disappears after addition of the stopping solutionand will neither affect the sensitivity nor the stability of the substrate. Stability…
The TMB PLUS has a higher activity level, resulting in a higher sensitivity and a reduced incubation time than found using the TMB ONE. TMB is a substrate for HRP. The mono-component, liquid TMB solution combines TMB, substrate buffer and hydrogen peroxide for a safe, ready-to-use, one step, chromogen system.The product does not contain harmful solvents. Recommended for ELISA procedures, liquid TMB yields a soluble blue end pr…
This new type of TMB with a red indicator combines all the important qualities of TMB PLUS, eliminating possible mistakes during pipetting.Further the red colour may be useful in automatic analyzers giving the warning "Reagent low" when it is time for filling up the substrate. The red indicator disappears after addition of the stopping solution and will neither affect the sensitivity nor the stability of the substrate. Stabili…
TMB is a substrate for HRP. Our mono-component, liquid TMB solution combine TMB, substrate buffer and hydrogen peroxide for a safe, ready-to-use, one step, chromogen system.The products do not contain harmful solvents. The TMB X-tra is a unique TMB substrate with an excellent resistancy and the highest sensitivity.
This TMB X-tra with red indicator combines all the important qualities of TMB X-tra eliminating possible mistakes during pipetting.Further the red colour may be useful in automatic analyzers giving the warning “Reagent low” when it is time for filling up the substrate. The red indicator disappears after addition of the stopping solution and will neither affect the sensitivity nor the stability of the substrate.
