Cell Death / Health Assays
Rat mammary gland undergoes programmed cell death processes during the tissue regression that follows lactation. Apoptotic bodies, which can be seen by routine histologic staining, can be specifically stained by techniques that localize DNA strand breaks in situ. The specificity of staining for strand breaks can thus be correlated with the morphological changes that define the process of apoptosis.
APOPTAG® Plastic Coverslips are designed to be custom fitted to the specific APOPTAG® kit application.
The ApopTag® Peroxidase In Situ Oligo Ligation Kit specifically detects apoptosis by staining only cells that contain double-stranded breaks that are blunt-ended or have a one base 3' overhang (cells containing nicked, gapped, 3'-recessed, 3'-overhanging ends longer than one base and single-stranded ends are not detected). The high analytic specificity of the ApopTag® ISOL Kit minimizes background due to non-specific labeling…
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's Caspase-3 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The free pNA can be quantified using a s…
Chemicon's Quick Apoptotic DNA Ladder Detection Kit provides a fast and easy means for detection of DNA fragmentation in apoptotic cells. Unlike other similar kits which require 1-2 days to finish the procedure, the new detection method requires less than 90 minutes to prepare DNA in a single tube, without the need for extraction or the use of columns. DNA fragmentation can be easily visualized by agarose gel electrophoreses.…
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's Caspase-8 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate IETD-pNA. The free pNA can be quantified using a s…
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's Caspase-9 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the LEHD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate LEHD-pNA. The free pNA can be quantified using a spectropho…
The CHEMICON Glutathione Detection Kit is a quick method for detection of intracellular levels of reduced glutathione (GSH). Reduction in levels of GSH may be an early indicator of apoptosis.
MTT is a pale yellow substrate that is cleaved by living cells to yield a dark blue formazan product. This process requires active mitochondria, and even freshly dead cells do not cleave significant amounts of MTT. The colorimetric assay described below can be used for either proliferation or complement-mediated cytotoxicity assays.
CHEMICON®'s Osteogenesis Quantitation Kit provides all the necessary reagents and a protocol to enable quantification of osteogenesis using a standard plate reader. This product is useful for studying the effects of growth factors, drugs and toxic agents on bone formation and for screening candidate osteogenic pharmaceuticals. Also, studies examining the intracellular signaling pathways regulating osteoblast differentiation ca…
