Reagents
Phosphoramidites for DNA oligonucleotide synthesis, gene synthesis, and more. Features of DNA Phosphoramidites: Amine functions are protected by either a benzoyl or isobutyryl group {dA(Bz), dC(Bz), dG(Ib)}. The recommended procedure for cleavage and deprotection for standard oligonucleotide synthesis is incubation in a concentrated ammonia solution for 8 hours at 55 °C. The purities of manufactured nucleoside phosphora…
Phosphoramidites for RNA oligonucleotides synthesis and more.Features of RNA Phosphoramidites: Amine groups are protected by “either a benzoyl or isobutyryl group {dA(Bz), dC(Bz), dG(Ib)}. Cleavage and deprotection procedures for RNA synthesis is similar to DNA synthesis, with just an additional step to remove the 2'-OH protecting group. The 2'-OH group is protected by a tert-butyldimethylsilyl (TBDMS) group to prevent de…
Amino linker phosphoramidites for the functionalization of the 5'-end during oligo synthesisFeatures• Amino linker products are coupled with standard synthesis protocols, identical to the coupling of DNA monomer phosphoramidites.• 5'-Amino phosphoramidite is used to add a functional amine group at the 5'- or 3'- end of the oligonucleotides. The primary amino group can be used for subsequent conjugation of a variety of repo…
Spacer phosphoramidites for oligonucleotide synthesis. Wide range of lengthsA wide selection of linker lengths available: C3, C4, C6 and C12Features• The spacer phosphoramidite are used to put spacer arms into oligonucleotides.C3, C6 and C12 spacer phosphoramidite contain an aliphatic linker which can be added to oligo requiring longer spacer arms. In general terms, spacer phosphoramidites are used during oligonucleotide s…
Phosphoramidites for nucleic acid labeling, microarray labeling, and moreFeatures• Oligonucleotides modified with fluorescein derivatives can be used for genomic research, molecular diagnostics and molecular biology.• t-butylamine/H2O/MeOH is recommended for cleavage and deprotection steps of TAMRA modified oligos.• dI-phosphoramidite can perform as an universal base as it hybridizes with any of the four bases.Technical Info…
High load CPG prepackaged Universal solid support for DNA SynthesisControlled Pore Glass (CPG) is the gold standard for solid phase oligonucleotide synthesis, with features such as high surface area, tightly controlled pore size and chemical inertness.Our High load CPG for DNA synthesis is available with or without phosphoramidites and can be purchased for standard synthesis sizes or for long-mer synthesis.Features• Utilizes C…
