Solid-phase extraction (SPE) is used for clean-up, extraction and concentration of semi-volatile or non-volatile analytes from complex mixtures including blood, urine and food samples. Multiple formats are available for conducting SPE, including prepacked SPE cartridges, disks and microplates, as well as SPE sorbent powders for manual packing. SPE systems can be used to automate the process and extract multiple samples at once. Solid-phase microextraction (SPME) and supported liquid extraction (SLE) products are also available in the same formats. Find the best SPE, SLE and SPME equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Functional groups, such as octyl (C8) and octadecyl (C18), can be bonded to the silica surface to provide non polar interactions. Each of these sorbents exhibits unique properties of retention and selectivity for a particular analyte. The choice of which sorbent is best for a particular method will be influenced by the percent recovery of analyte from the sample matrix and the cleanliness of the resulting chromatography.
Functional groups, such as octyl (C8) and octadecyl (C18), can be bonded to the silica surface to provide non polar interactions. Each of these sorbents exhibits unique properties of retention and selectivity for a particular analyte. The choice of which sorbent is best for a particular method will be influenced by the percent recovery of analyte from the sample matrix and the cleanliness of the resulting chromatography.
This chemistry promotes high retention of a wide range of analytes, reducing method development time and facilitating a generic approach to sample preparation.
Functional groups, such as octyl (C8) and octadecyl (C18), can be bonded to the silica surface to provide non polar interactions. Each of these sorbents exhibits unique properties of retention and selectivity for a particular analyte. The choice of which sorbent is best for a particular method will be influenced by the percent recovery of analyte from the sample matrix and the cleanliness of the resulting chromatography.
SDB-XC is a poly(styrenedivinylbenzene) copolymer used as a reversed phase sorbent for solid phase extraction. SDB-XC is not bonded to silica but is a 100% copolymeric particle that is spherical, porous and cross-linked. It does not exhibit the secondary cationic (silanol) interactions and pH limitations common to bonded silica sorbents, so it offers more predictable and reproducible reversed phase interaction.
The Empore™ High Performance Extraction Disk Plate differs in design from plates offered by other manufacturers and requires a specific vacuum manifold. Manifolds supplied by different plate manufacturers are specific to the design of their plates and cannot generally be used with plates from other manufacturers. In some cases, the use of spacers to raise/lower the height of a collection device inside the manifold may overco…
Replacement gaskets are available with the vacuum manifold assembly. The gasket can become worn over time and the seal between the plate and the manifold will be affected. This may result in poor vacuum pressure and negatively effect flow rates. The gasket should be checked regularly and replaced when it becomes worn.
Empore Filter Aid 400 has been shown to improve flow rates and recoveries for separation and filtration of water samples containing suspended solids. When placed on top of the Empore™ Extraction Disk at a depth of about 1 cm, Empore™ Filter Aid 400 acts as depth filter to inhibit the migration of suspended solids to the surface of the disk. This reduces extraction time and improves rates and recovery of analytes.
Spark has introduced the second edition of HySphere™ C8 End-Capped cartridges. We have selected a new perfect Spherical sorbent material with a narrow particle size distribution. This will create less back pressure during operation and allows you to run higher flow rates compared to our current HySphere™ C8 (EC). The new cartridge has the same excellent characteristics such as high extraction capacity and selectivity
Versatile polymeric material for retention of polar and non-polar analytes • Exceptional recoveries for polar and non-polar analytes • High and consistent recoveries • High capacity material • Fast and easy sample preparation method development • pH stable 0-14 HyperSep Retain PEP (polar enhanced polymer) is a high purity, highly porous polystyrene DVB material modified with urea functional groups to give balanced retention of…
Thermo Scientific Online SPE Products have been designed to clean-up and concentrate target analytes in minutes. The products are available in different formats and utilize popular HyperSep SPE materials such as the HyperSep™ Retain polymeric range of materials and HyperSep Hypercarb™ for polar analyte retention.
Symbiosis™ Pharma is Spark Holland's unique solution for integrated online SPE-LC-MS automation (XLC-MS). The heart of the matter in XLC-MS, is where the autosampler injects at a controlled temperature, raw bio-fluids directly on a fresh disposable SPE cartridge. The small particle sorbent assures a clean extract, a very high sensitivity and reliable results. Online elution to the LC-MS cuts the number of sample prep steps in…
SCP Science DigiSEP Blue SPE Cartridges A general purpose, single use, cation removal cartridge. DigiSEP Blue from SCP Science is a highly selective exchange cartridge is ideal for retaining transition metals in samples that contain high concentrations of potassium and sodium as in sea water. DigiSEP Blue has an aminodiacetate group that acts as a chelating agent to a variety of metallic ations in solution providing a mean t…
High recovery sample preparation for drug analysis and screening: LiChrolut® TSC is a selective and efficient solid phase extraction sorbent designed for clean-up of pharmaceutical products and drugs obtained from blood, plasma and urine. LiChrolut® TSC belongs to the family of the so-called “mixed-mode phases”. The combination of a reversed-phase and a cation exchange mechanism gives rise to retention of the analyte on the st…
For reproducible sample preparation: LiChrolut® CN and NH2 are medium polar phases for solid phase extraction, which produces excellent reproducibility of sample preparation. This results a combination of high chemical stability and guaranteed capacity. LiChrolut® CN and NH2 are suitable for both polar and non-polar extractions. These medium polar phases have, in addition to a short-chained propyl-spacer, a polar functional gr…
Highest capacity for solid phase extraction: LiChrolut® EN saves time and money in solid-phase extraction as less time is required for conditioning and drying. In addition, only a little solvent is required for elution of the analytes from the cartridge bed. The reduced quantity of solvent leads to a lower degree of contamination and to an increase in detection sensitivity. This improves the analysis of the relevant extracted…
Using the LiChrolut® extraction unit simplifies solid-phase extraction and makes it more rapid and reliable. The drying attachment speeds up the drying process under controlled conditions. All the individual steps associated with solid-phase extraction can be carried out using the LiChrolut® extraction unit.
Solid-phase extraction with LiChrolut® is a reliable and rapid route to successful sample preparation and the selective extraction of compounds from a complex sample. Three different modified SPE sorbents are available for non-polar solid-phase extraction: LiChrolut® RP-18 endcapped, LiChrolut® RP-18 and LiChrolut® RP-select B. The difference in polarities are clearly described by the adsorption characteristics. This can save…
A unique high performance ion exchange system for solid phase extraction: LiChrolut® tentacle ion exchanger improves the mass transfer and the recoveries in ion exchange solid phase extraction. Small elution volumes, very high chemical stability and very high capacity are also reasons for the superiority of LiChrolut® SCX and SAX. In contrast to conventional ion exchangers, tentacle functional exchange groups do not lie in def…
LiChrolut® Si 60 is a high purity sorbent for solid phase extraction. Highly purified LiChrolut® Si 60 on the basis of non-modified silica with a pore diameter of 6 nm is available for conventional polar extractions.
Solid-phase extraction for increased productivity: LiChroPLATE® microtiter plates improve the sample throughput and speed of sample preparation. LiChroPLATE® enables solid-phase extraction to be carried out with considerable convenience and flexibility. From sample application with multi-channel pipettors to complete automation of the sample preparation process. Only in this way high sample throughputs can be achieved. LichroP…
Enhanced selectivity for polar analytes: LiChroPLATE® EN microtiter plates for solid-phase extraction shortens sample preparation time up to 75% compared with other polymer phases. By using LiChroPLATE® EN in combination with an 8-channel pipettor, it is possible to extract 96 samples in less than half an hour. EN stands for enhanced selectivity with respect to all polar analytes – whether basic, neutral or acidic. Even for la…
Solid-phase extraction for increased productivity: LiChroPLATE® RP-18 microtiter plates for solid-phase extraction enable ultra-rapid sample preparation with excellent recovery rates and high precision. Up to 96 samples can be processed simultaneously, which means a significant reduction in the time needed for sample preparation and consequent savings on purchasing and disposal costs. The batch-to-batch reproducibility of LiCh…
For direct in-line sample preparation of untreated bio-fluids: LC-integrated sample preparation with LiChrospher® ADS is less expensive, less time consuming and more precise in comparison to conventional off-line sample preparation. LiChrospher® ADS allows the direct extraction and enrichment of hydrophobic, low molecular analytes from untreated samples such as haemolysed blood, plasma, serum, milk, salivary fluid, fermentatio…
Designed for use with the Bioneer AccuPrep® 96-well plate series - handles 96 samples simultaneously for high throughput DNA extraction. Simply attach to a vacuum pump for easy processing of all AccuPrep 96-well plate DNA extraction kits.
