Cell-Based Assays Products & Reviews

Multi-analyte controls in diluent that mimics human CSF. The concentrations of the analytes are provided in the lot-specific certificate of analysis.

For use to enrich for specific regions of chromatin that are immunoprecipitated with a specific antibody for a chromatin protein. Works with all eukaryotic cell types. Detection of the gene or promoter of interest in immunoprecipitated chromatin must be empirically determined by the researcher. Quantitative PCR detection is recommended.

R&D Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit - R&D Mouse IL-1/IL-1F2 Immunoassay for the quantitative determination of mouse interleukin 1 beta (IL-1ß) concentrations in cell culture supernates and mouse serum. The R&D Quantikine Mouse IL-1 Immunoassay is a 4.5 hour solid phase ELISA. This R&D Quantikine assay employs the quantitative sandwich enzyme immunoassay technique. Features of the R&D Quantikine ELISA Kit inclu…

More than 18,000 studies have been published citing the use of the yeast two-hybrid system to investigate protein-protein interactions, and Clontech’s Matchmaker™ Systems have been by far the most popular. Matchmaker™ Gold Systems are the highest performing incarnations of our Matchmaker product line, due to a combination of a new yeast strain (Y2HGold), stringent reporters, easy to use libraries, high-level expression vectors…

The G-LISA™ assay uses a 96-well plate coated with RBD domain of Rho-family effector proteins. The active GTP-bound form of the Rho-family protein, but not the inactive GDP-bound form, from a biological sample will bind to the plate. Bound active Rho-family protein is then detected by incubation with a specific primary antibody followed by a secondary antibody conjugated to HRP. The signal is then developed with OPD or chemilu…

Each VECTASTAIN® ABC kit utilizes the same preformed Avidin: Biotinylated enzyme Complex (ABC) principle and all offer low backgrounds and an amplification of signal compared to conven-tional covalent conjugates. The high sensitivity reported for this technique likely resides in either the form or the number of active enzyme molecules associated with the complex and the rapid, irreversible interaction of the complex with bio…

Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…

Chemotaxis assays are ideal for assessing the effects of pharmacological compounds on the motility of tumor cells, and for analyzing the migratory capacity of multiple cell lines in parallel. The format options enable large-scale screening and quantitative comparison of multiple samples in a user friendly, sensitive, HTS system. Quantitative fluorescent method increases sensitivity 10-fold over similar assays on the market…

Efficient Lysis of E. coli Cultures; No Centrifugation or Mechanical Disruption Needed FastBreak™ Cell Lysis Reagent is provided as a 10X concentrate and contains a proprietary nonionic detergent to facilitate lysis. Add the reagent directly to E. coli cultures. Following a brief incubation, the cells are disrupted, and the protein  released. Recombinant proteins can be directly screened in the cell extract or purified by t…

Cost effective high throughput screen for anti-cancer compounds. Basic research to measure compound IC50s and specificity for tubulin. Screening proteins for effects on tubulin polymerization activity. Teaching aid for undergraduate/graduate class in pharmacology. This assay is an economical one step procedure for determining the effects of drugs or proteins on tubulin polymerization. It is an adaptation of an assay o…