Enzymes
Thermo Scientific X-Gal (5-bromo-4-chloro-3-indoyl-ß-D-galactopyranoside) is a chromogenic substrate used to identify recombinant plasmids. In the presence of X-Gal and IPTG, lac+ bacterial colonies appear blue, whereas recombinant colonies appear white.
Axygen X-Gal IPTG spray is a convenient ready-to-use solution of optimal quantities of IPTG, X-Gal and stabilizers presented in a spray bottle for easy, accurate and consistent screening of Blue/White colonies. Advantages of Axygen X-Gal-IPTG Spray include:• Easy, accurate and convenient. • Sprays deliver optimal concentration and volume consistently for consistent and accurate results. • No need to weigh X-Gal and IPTG and…
Finnzymes’ novel PowerCut™ Dicer produces a pool of 25–27 nucleotide-long siRNA molecules from any dsRNA. This efficient endoribonuclease originating from Giardia intestinalis outperforms all other siRNA producing enzymes by cleaving the substrate with 100 % efficiency yet leaving the resulting siRNA intact. The high quality siRNA produced is ideal for gene silencing studies, for example. Pooled siRNAs are also preferred over…
The TMB SENS is the latest TMB substrate released from our R&D team. The sensitivity of this TMB product is superior in todays marketplace. When comparing the TMB SENS with TMB ONE the OD-values more than double. Recommended for ELISA procedures, liquid TMB yields a soluble blue end product read at 655 nm. For all our TMB products superior stop stability is achieved by a 0,3 M H2SO4 resulting in a yellow color read at 450 nm…
Top DNA polymerase is a novel thermostable DNA polymerase that is more processive than Taq DNA polymerase. In fact, the extension rate of Top DNA Polymerase is > 3 x that of Taq DNA Polymerase! Top DNA Polymerase can be used for a variety of PCR applications (including TA cloning) and is a robust enzyme for everyday PCR. It contains no proofreading or 5’-3’ Exonuclease activity.Features and Benefits• Fast: More than three tim…
Get more cDNA in less time with CycleScript Reverse Transcriptase. CycleScript is a versatile reverse transcriptase - applicable to both conventional Reverse Transcription and Cyclic Reverse Transcription (Cyclic RT, patent pending – for cDNA amplification). It features high activity across a wide range of temperatures from 37 to 55°C; therefore, reverse transcription is carried out like PCR. The Cyclic RT reaction is composed…
Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase is an RNA-dependent DNA polymerase. This enzyme is able to use an RNA molecule as a template and synthesize a double-strand DNA. M-MLV Reverse Transcriptase is isolated from a E.coli strain containing a recombinant clone. It is ideal for use of first-strand synthesis cDNA from RNA molecules and for cDNA synthesis for Reverse Transcriptase PCR and qRT-PCR.
