Polymerase chain reaction (PCR) kits and thermal cyclers are used for the <i>in vitro</i> amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
The enzyme dNTPack comprises the GC-RICH PCR System and a ready-to-use solution of PCR-Grade Nucleotides.The GC-RICH PCR System, a blend of Taq DNA Polymerase and a proofreading polymerase, powers through templates that are difficult or impossible to amplify with other polymerases and other blends of polymerases. The enhanced processivity of the blend and the unique GC-RICH Resolution Solution combine to deliver superior perfo…
The GC-RICH PCR System, a blend of Taq DNA Polymerase and a proofreading polymerase, powers through templates that are difficult or impossible to amplify with other polymerases and other blends of polymerases. The enhanced processivity of the blend and the unique GC-RICH Resolution Solution combine to deliver superior performance – especially from problematic templates. PCR Amplification of difficult templates up to 5 kb…
The enzyme dNTPack comprises the Expand High FidelityPLUS PCR System and a ready-to-use solution of PCR-Grade Nucleotides. Expand High FidelityPLUS PCR System is a next-generation blend consisting of Taq DNA Polymerase and a novel proofreading protein, isolated and characterized by Roche Applied Science. This protein mediates proofreading activity but has no polymerase activity itself. Choose Expand High FidelityPLUS PCR Syste…
The Expand High Fidelity PCR System is an enzyme blend containing Taq DNA Polymerase and a polymerase with proofreading activity. Expand High Fidelity PCR System delivers superior results with two-fold higher yield and three-fold greater fidelity compared to Taq DNA Polymerase. Choose Expand High Fidelity PCR System for the most sensitive PCR results for genomic targets up to 5 kb in length. PCR RT-PCR Benefits The E…
The enzyme dNTPack comprises the Expand High Fidelity PCR System and a ready-to-use solution of PCR-Grade Nucleotides. The Expand High Fidelity PCR System is an enzyme blend containing Taq DNA Polymerase and a polymerase with proofreading activity. Expand High Fidelity PCR System delivers superior results with two-fold higher yield and three-fold greater fidelity compared to Taq DNA Polymerase. Choose Expand High Fidelity PCR…
The enzyme dNTPack comprises the Pwo SuperYield DNA Polymerase and PCR-Grade Nucleotides in a ready-to-use solution.Pwo SuperYield DNA Polymerase is ideal for high-fidelity amplification of DNA up to 3 kb. Pwo SuperYield DNA Polymerase combines the recombinant enzyme Pwo DNA Polymerase with a newly optimized buffer system. This new buffer system enhances the enzymatic properties of the polymerase, resulting in higher yields of…
The enzyme dNTPack comprises Taq DNA Polymerase and a ready-to-use solution of PCR-Grade Nucleotides. Taq DNA Polymerase is the recombinant form of the enzyme from the thermophilic eubacterium Thermus aquaticus BM, expressed in E. coli. The enzyme is highly purified and is free of nonspecific endo- and exonucleases. It is a highly processive 5'-3' DNA polymerase that lacks 3'-5' exonuclease activity. PCR RT-PCR Primer…
DyNAmo™ Probe qPCR Kit is a ready-to-use 2x master mix for highly specific quantitative PCR. The master mix contains all reagents needed for qPCR. Only template DNA, PCR primers and probe need to be added by the user.The 2x master mix includes the efficient hot start Thermus brockianus DNA polymerase. ROX™ passive reference dye is also supplied with the kit in a separate tube for instruments that require ROX normalization. DyN…
Phusion™ RT-PCR Kit is a complete two-step kit designed for high-fidelity RT-PCR. The kit contains the full set of reagents required for performing cDNA synthesis and PCR in two steps. In the first step, a variety of RNA templates and all priming options may be used for efficient synthesis of cDNA with M-MuLV Reverse Transcriptase.In the second step, Phusion Hot Start High-Fidelity DNA Polymerase is used for cDNA amplification…
Phusion™ Flash High-Fidelity PCR Master Mix is a 2x master mix enabling the use of extremely short PCR protocols without compromising neither the fidelity nor the yield in the reaction. Phusion Flash DNA Polymerase used in the master mix is a proofreading DNA polymerase modified from Phusion Hot Start High-Fidelity DNA Polymerase. Phusion Flash High-Fidelity PCR Master Mix enables the use of extremely short cycling protocols w…
