RNAi Reagents
The MISSION pLKO.1-puro Control Vector is a lentivirus plasmid vector. The vector does not contain an shRNA insert and is useful as a negative control in experiments using the MISSION shRNA library clones.
Developed at the Broad Institute, the MISSION ® TRC3 human whole genome pooled ORF library consists of over 17,000 unique ORF clones, encoding for over 14,000 genes used for overexpression screening
The MISSION TRC3 ORF GFP Control Vector is a lentivirus plasmid vector. The vector contains an ORF insert expressing TurboGFP™, and is useful as a positive (or negative control) in experiments using the MISSION TRC3 LentiORF Library Collection in arrayed or pooled format
siPORT NeoFX’s lipid-based formulation enables the transfection of siRNA into adherent cells as they are subcultured — without increased cytotoxicity. From start to finish, successful gene silencing experiments can be completed in as little as 24 hours. This streamlined protocol can be adapted to a wide range of cells and experimental designs, including high-throughput applications.
Ambion Anti-miR™ miRNA Inhibitors are chemically-modified, single-stranded nucleic acids designed to specifically bind to and inhibit endogenous miRNA molecules. These ready-to-use inhibitors can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs, and enable detailed study of miRNA biological effects.
Silencer Pre-Designed siRNAs are Ambion-designed siRNAs available for all human, mouse, and rat gene targets in the RefSeq database. These siRNAs are designed for maximum potency and specificity using a highly effective and extensively tested algorithm. Each siRNA is synthesized to the highest quality standards and is provided with full sequence information. Silencer Validated siRNAs are Ambion-designed siRNAs that have been…
Ambion Silencer Select Pre-Designed and Validated siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNA with unrivaled efficacy, potency, and specificity. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data.
Ambion Silencer® In Vivo Ready siRNAs undergo the additional purification and testing necessary for introduction of siRNA into animals. After HPLC purification and annealing, each siRNA is dialyzed to remove salt, sterile filtered, and endotoxin tested. All Silencer Pre-Designed and Validated siRNAs are available in this format, as are Custom Synthesized siRNAs.
Abmion Validated GAPDH Positive Control siRNA and non-targeting Negative Control siRNAs are available for human, mouse and rat with the same chemical modifications for enhanced efficacy as in other Ambion Silencer Select siRNAs.
