Oligonucleotides Products & Reviews

Oligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.

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ATPlite 1Step

Revvity

ATP luminescence assay for quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells. The 1step luciferase ATP assay format only requires one addition step, and can be used for continuous processing.

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Thermo Scientific Random Decamers

Thermo Fisher Scientific

Thermo Scientific PCR reagents meet high quality standards to generate accurate results from even the most challenging molecular biology applications. Random Decamers are truly random oligo deoxyribonucleotides that can be used to create a pool of cDNAs for subsequent applications, thus providing maximum flexibility.

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Thermo Scientific Random Hexamers

Thermo Fisher Scientific

Thermo Scientific Random Hexamers are truly random oligo deoxyribonucleotides. Hexamers are ideal primers for mRNA with or without a poly-A tail. Hexamers bind more often than decamers, giving higher yields and increased sensitivity when amplifying short fragments.

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DNA Phosphoramidites

Bioneer

Phosphoramidites for DNA oligonucleotide synthesis, gene synthesis, and more. Features of DNA Phosphoramidites: Amine functions are protected by either a benzoyl or isobutyryl group {dA(Bz), dC(Bz), dG(Ib)}. The recommended procedure for cleavage and deprotection for standard oligonucleotide synthesis is incubation in a concentrated ammonia solution for 8 hours at 55 °C. The purities of manufactured nucleoside phosphora…

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RNA Phosphoramidites

Bioneer

Phosphoramidites for RNA oligonucleotides synthesis and more.Features of RNA Phosphoramidites: Amine groups are protected by “either a benzoyl or isobutyryl group {dA(Bz), dC(Bz), dG(Ib)}. Cleavage and deprotection procedures for RNA synthesis is similar to DNA synthesis, with just an additional step to remove the 2'-OH protecting group. The 2'-OH group is protected by a tert-butyldimethylsilyl (TBDMS) group to prevent de…

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