Enzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
With the ChemiKine assay system, sheep polyclonal antibodies generated against human NT-3 are coated onto a microplate and are used to capture NT-3 from a sample. NT-3 specific, biotin conjugated, mouse monoclonal antibodies detect the captured NT-3. After addition of streptavidin-enzyme, substrate and stop solution the amount of NT-3 is determined. The standard curve demonstrates a direct relationship between Optical Density…
With the ChemiKine NGF assay system, sheep polyclonal antibodies generated against mouse NGF are coated onto a microplate and are used to capture NGF from a sample. ChemiKine NGF kit is designed to measure the amount of NGF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from mouse and rat. There are enough reagents included in this kit for two 96-well immuno-assay…
ChemiKine™ Brain Derived Neurotrophic Factor (BDNF) kit is a sandwich enzyme immunoassay (EIA), which measures BDNF. The kit will measure BDNF from human and rat. ChemiKine BDNF kit is designed to measure the amount of BDNF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from human and rat. There are enough reagents included in this kit for two 96-well immuno-assay…
ChemiKine™ Pigment Epithelium-Derived Factor (PEDF) kit is a sandwich stripwell-type enzyme immunoassay (EIA), which measures PEDF. The kit will measure PEDF of vitreous fluid of human origin. With the ChemiKine™ PEDF assay system, a mouse monoclonal antibody generated against human PEDF is coated onto a microplate and is used to capture PEDF from a sample. A second, biotinylated PEDF-specific mouse monoclonal antibody detects…
The QuantiMatrix™ ELISA kit for the determination of soluble human fibronectin provides sensitivity down to 10-20 ng/mL as determined from culture supernatants and purified protein samples. The assay is in the format of a competitive inhibition ELISA. Human fibronectin is pre-coated on the wells with the exception of row H, which serves as a reference.
The VCAM-1 ELISA is an enzyme-linked immunosorbent assay for quantitative detection of soluble Vascular Cell Adhesion Molecule-1 in cell culture supernatants, serum, plasma, or other biological fluids. The VCAM-1 ELISA is for research use only. Not for use in diagnostic procedures.
The MMP-9 immunoassay kit utilizes antibodies immobilized on a bead matrix, in combination with enzyme-labeled antibodies, directed against different antigenic sites on the same MMP-9 molecule. The MMP-9 immunoassay kit is useful for the determination of MMP-9 (pro-MMP-9) levels in fresh human plasma and conditioned medium of human cells. Human serum is not an acceptable sample for evaluation.
EMD Millipore’s Human Amyloid β40 ELISA kit is used for the measurement of Amyloid beta 40 in brain samples and for other tissue samples in a 96-well format. This assay is for research use only and appropriate for the in vitro detection of human Amyloid β40 peptides in brain samples from e.g. Guinea pig, transgenic hAmyloid mice and cell extracts.
EMD Millipore’s Human Amyloid β42 Brain ELISA kit is used for the measurement of Amyloid beta 42 in brain samples and for other tissue samples in a 96-well format. This assay is for research use only and appropriate for the in vitro detection of human Amyloid β42 peptides in brain samples from e.g. Guinea pig, transgenic hAmyloid mice and cell extracts.
Amylin is a 37 amino acid hormone that is co-secreted with insulin from pancreatic beta cells in response to hyperglycemia.
Adiponectin is identified as a 30 kDa adipokine that is secreted from the adipose tissue into circulating blood and influences systemic metabolism.
Amylin is a 37 amino acid hormone that is co-secreted with insulin from pancreatic beta cells in response to hyperglycemia.
This unique Human Insulin Elisa kit is animal serum free–the monoclonal antibodies are harvested from clones grown in culture medium devoid of any animal protein.
Proinsulin, the precursor of insulin and C-peptide, is made by the beta cells of the pancreas. This kit measures only the intact form of human proinsulin
Proinsulin, the precursor of insulin and C-peptide, is made by the beta cells of the pancreas. This kit measures the intact and intermediate metabolic forms of human proinsulin.
This Human Pancreatic Polypeptide (PP) ELISA kit is used for the non-radioactive quantification of Human PP in human serum, plasma, tissue extract and cell culture samples. One kit is sufficient to measure 39 unknown samples in duplicate
Adiponectin is identified as a 30 kDa adipokine that is secreted from the adipose tissue into circulating blood and influences systemic metabolism.
Serum Amyloid A3 (SAA-3) is an acute phase protein produced in the liver. In response to injury, granulocytes and macrophages secrete cytokines which modulate the production of acute phase proteins. Many of these proteins, such as SAA-3, α1-acid glycoprotein (AGP), lipoclain 24P3, plasminogen activator inhibitor-1 (PAI-1) and PTX3 are secreted from adipocytes and are elevated in various metabolic disease states.
Type II collagen is the major collagen found in cartilage and is expressed in two forms: IIA and IIB.
Adiponectin is identified as a 30 kDa adipokine that is secreted from the adipose tissue into circulation blood and influences systemic metabolism.
Rat/Mouse Ghrelin (Active) ELISA kit for the quantification of active ghrelin in rat and mouse serum or plasma samples. Each kit is sufficient to measure 39 unknown samples in duplicate.
Leptin is synthesized in adipocytes as a 16 kDa molecule and the circulating level is directly proportional to the total amount of fat in the body.
EMD Millipore is pleased to offer the Rat/Mouse C-Peptide 2 ELISA kit for the quantification of C-peptide 2 in rat and mouse serum and plasma samples. Each kit is sufficient to measure 39 unknown samples in duplicate.
Growth Hormone (GH) is a 23 kDa circulating pituitary hormone, Rat GH shares approximately 97% homology with mouse GH and 65% homology with human GH in its protein sequence.
GIP, also known as gastric inhibitory polypeptide, or glucose-dependent insulinotropic polypeptide, is a 42 amino acid peptide hormone synthesized in and secreted from K cells in the intestinal epithelium.
