Oligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.
Amino linker phosphoramidites for the functionalization of the 5'-end during oligo synthesisFeatures• Amino linker products are coupled with standard synthesis protocols, identical to the coupling of DNA monomer phosphoramidites.• 5'-Amino phosphoramidite is used to add a functional amine group at the 5'- or 3'- end of the oligonucleotides. The primary amino group can be used for subsequent conjugation of a variety of repo…
Spacer phosphoramidites for oligonucleotide synthesis. Wide range of lengthsA wide selection of linker lengths available: C3, C4, C6 and C12Features• The spacer phosphoramidite are used to put spacer arms into oligonucleotides.C3, C6 and C12 spacer phosphoramidite contain an aliphatic linker which can be added to oligo requiring longer spacer arms. In general terms, spacer phosphoramidites are used during oligonucleotide s…
Phosphoramidites for nucleic acid labeling, microarray labeling, and moreFeatures• Oligonucleotides modified with fluorescein derivatives can be used for genomic research, molecular diagnostics and molecular biology.• t-butylamine/H2O/MeOH is recommended for cleavage and deprotection steps of TAMRA modified oligos.• dI-phosphoramidite can perform as an universal base as it hybridizes with any of the four bases.Technical Info…
High load CPG prepackaged Universal solid support for DNA SynthesisControlled Pore Glass (CPG) is the gold standard for solid phase oligonucleotide synthesis, with features such as high surface area, tightly controlled pore size and chemical inertness.Our High load CPG for DNA synthesis is available with or without phosphoramidites and can be purchased for standard synthesis sizes or for long-mer synthesis.Features• Utilizes C…
For routine applications, Bioneer offers standard custom oligos with no modification, Bio-RP purification, provided in a tube format. The chart in the ordering information tab shows pricing at various synthesis scales, but please contact your sales representative to learn about any institutional or volume discounts we might have in place for our oligonucleotides.
A total capacity of > 30,000 oligonucleotides per day gives Bioneer the opportunity to address the needs of researchers that require very large numbers of oligonucleotides. In-house production of raw materials along with proprietary high throughput oligo synthesis and purification systems result in much higher quality oligonucleotides at significantly lower cost. Bioneer understands that high quality and low cost is especially…
Bioneer provides the ideal tools for your specialized applications. With the advent of technologies such as real-time detection and quantification, as well as mass-scale genotyping projects, more and more scientists require the use of modified oligos. All common Oligo modifications are available. Our fluorogenic oligos for qPCR, are unsurpassed in quality, and priced for value. In addition to the modifications listed in our or…
Extendamers™ are oligonucleotides from 130 - 200 bases. Bioneer specializes in long oligo synthesis and provides them with Bio-RP or PAGE purification. As with all Bioneer Oligonucleotides, Extendamers are QC tested by MALDI-TOF after oligo synthesis, ensuring the highest quality products for your research needs. Bio-RP (or PAGE) purification also ensures you have only full length oligonucleotides for your experiments. Use Ext…
Extendamers™ are oligonucleotides from 130 - 200 bases. Bioneer specializes in long oligo synthesis and provides them with Bio-RP or PAGE purification. As with all Bioneer Oligonucleotides, Extendamers are QC tested by MALDI-TOF after oligo synthesis, ensuring the highest quality products for your research needs. Bio-RP (or PAGE) purification also ensures you have only full length oligonucleotides for your experiments. Use Ext…
Bioneer’s production facilities can accommodate large scale oligonucleotide synthesis orders ranging from milligrams to tens of grams of the purest DNA and RNA oligos, including both standard and modified. A large portion of our bulk oligo customers are involved in research requiring antisense oligos, which has opened the door to new approaches in the development of pharmaceuticals and target validation. Some of the frequently…
