Polymerase Products & Reviews

The engineered PfuUltra II Fusion HS DNA Polymerase provides the worlds most accurate copies and the fastest extension times possible. PfuUltra II Fusion HS DNA Polymerase is PfuUltra hotstart DNA polymerase coupled with a fusion polymerase technology and ArchaeMaxx PCR enhancing factor.  The industry standard for ultra high fidelity PCR providing the most accurate copies of DNA  Dramatically reduced extension times allow f…

These reactions buffers contain the optimal magnesium concentration for efficient PCR.  Ideal for optimal PCR performance  Standard 10x formulation  Optimized for use with specific PCR enzymes

T4 DNA Polymerase catalyzes the synthesis of DNA in the 5´→ 3´ direction and requires the presence of template and primer. This enzyme has a 3´→ 5´ exonuclease activity which is much more active than that found in DNA Polymerase I. Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5´→ 3´ exonuclease function. Source: Purified from a strain of E. coli that carries a T4 DNA Polymerase overproducing plasmid. Appl…

NovaTaq™ Hot Start DNA Polymerase is a chemically modified form of Taq DNA polymerase that is inactive at ambient temperature.

Reliable hot start Taq DNA Polymerase for sensitive detection of low abundance target DNA. Ideal for standard end point PCR and qPCR applications. Available in Glycerol-free, Triton-free, and high concentration formulations.

T7 RNA Polymerase catalyzes the 5′→3′ RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognizes the T7 promoter and terminator sequences with high specificity. Key features:  Reliable transcription 

PCRBIO Taq Mix is an affordable, versatile and robust 2x mix for all your everyday PCR applications including genotyping, screening and library construction. An enhanced 12-step polymerase purification strategy together with an optimised buffer system enable PCRBIO Taq Mix to amplify with the highest speed, yield and specificity.

PCRBIO HS Taq Mix is an advanced antibody-mediated hot start DNA polymerase mix designed for fast, highly specific and sensitive PCR. Whether you need a hot start assay for high-throughput, automated reaction setup or the detection of a low copy number template, PCR Biosystems offers you a robust industry-leading enzyme to meet your needs.

PCRBIO Classic Taq is a highly purified recombinant Taq DNA Polymerase for all your everyday PCR applications including genotyping, screening and library construction. The enzyme and buffer system allow for superior PCR performance on complex templates such as mammalian genomic DNA.

PCRBIO HS Taq DNA Polymerase is an advanced antibody-mediated hot start DNA polymerase designed for fast, highly specific and sensitive PCR. Whether you need a hot start assay for high-throughput, automated reaction setup or the detection of a low copy number template, PCR Biosystems offers you a robust industry-leading enzyme to meet your needs.

VeriFi® Mix is a versatile and robust high fidelity 2x ready-mix engineered for all PCR applications where greater sequence accuracy is required. Improved DNA binding and increased processivity result in shorter extension times, higher yields and the ability to amplify longer and more difficult targets. 

VeriFi® Hot Start Mix is a versatile and robust 2x proofreading ready-mix with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates.

VeriFi® Hot Start Mix Red is a versatile and robust 2x proofreading ready-mix with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates. The mix includes a convenient red dye for direct gel loading.

VeriFi® Hot Start Polymerase is a versatile and robust proofreading enzyme with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates.

PCRBIO HiFi Polymerase is our original high-fidelity enzyme, derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity. Several proprietary point mutations allow for significantly improved performance when compared with its native form. Together with advanced buffer chemistry this enzyme brings robust performance to the world of high fidelity PCR.

PCRBIO Ultra Polymerase has been engineered for the amplification of extremely difficult templates. Proprietary modifications that enhance processivity together with advanced buffer chemistry and hot start technology deliver outstanding performance whether your template is GC or AT-rich, low in abundance or contains PCR inhibitors.

PCRBIO Ultra Mix is a convenient 2x ready mix that has been engineered for the amplification of extremely difficult templates. Proprietary modifications that enhance processivity together with advanced buffer chemistry and hot start technology deliver outstanding performance whether your template is GC or AT-rich, low in abundance or contains PCR inhibitors.

PCRBIO Ultra Mix is a convenient 2x ready mix that has been engineered for the amplification of extremely difficult templates, with a red dye included for direct gel loading.

PCRBIO 1-Step Go RT-PCR Kit is a convenient, easy-to-use kit for fast and efficient cDNA synthesis and PCR in a single tube. The advanced buffer system, reverse transcriptase and hot start polymerase give highly specific and ultra-sensitive 1-step RT-PCR from any RNA template including mRNA, total RNA and viral RNA sequences.

IsoFast® Bst Polymerase is a recombinant form of the large fragment of Bst DNA polymerase, containing 5′-3′ polymerase activity and strand displacement activity.

IsoFast® Bst 1-Step Mix is a dual enzyme system for rapid and sensitive isothermal amplification of RNA targets in one step.

VeriFi® Library Amplification Mix is ideal for NGS library amplification workflows and challenging PCRs. Combining a powerful and robust proofreading enzyme, greatly reduced GC-dependent bias, and AptaLock™ hot start technology, this mix enables precise PCR, regardless the target you are sequencing.

IsoFast® Hot Start Bst Polymerase is the most recent and improved development of isothermal amplification technology, designed to deliver unparalleled specificity and speed in DNA amplification processes. This mix is suitable for applications such as multiple displacement amplification, isothermal amplification, loop mediated isothermal amplification (LAMP), molecular diagnostics and field diagnostics.

IsoFast® Hot Start Bst Mix is the most recent and improved development of isothermal amplification technology, designed to deliver unparalleled specificity and speed in DNA amplification processes. This mix is suitable for applications such as multiple displacement amplification, isothermal amplification, loop mediated isothermal amplification (LAMP), molecular diagnostics and field diagnostics.

IsoFast® Hot Start Bst Colour Mix is a colourimetric isothermal amplification enzyme formulation that combines IsoFast® Hot Start Bst Polymerase with a pH-based dye for rapid positive/negative screening.