Protein Expression
The pET E. coli expression system is a widely used in vivo bacterial expression system due to the strong selectivity of the bacteriophage T7 RNA polymerase for its cognate promoter sequences, the high level of activity of the polymerase, and the high efficiency of translation mediated by the T7 gene.
The VariFlex protein expression system offers vectors with unique tags that enhance the utility of E. coli as an expression host. Included in our tag offering are three different SET tags which increase protein solubility and the SBP purification tag for high-yield, pure proteins.
The pBK-CMV vector allows you to express proteins in both E. coli and mammalian model systems and saves you valuable time by not having to subclone into an additional vector. In mammalian cells, the expression is driven by the CMV promoter and is therefore constitutively expressed. In E. coli, the lac promoter drives high-level expression in the presence of the inducer IPTG, and allows minimal expression in the absence of i…
The Complete Control Inducible Mammalian Expression System is designed to provide the tightest control of expression available in mammalian cells. The system is based on a synthetic ecdysone-inducible receptor and a synthetic receptor recognition element that modulates expression of your gene of interest.
The LacSwitch II inducible mammalian expression system utilizes an improved vector system in which several elements of the lac operon have been modified for use in eukaryotic cells for the control of gene expression.
Epitope-tagging technology provides you a powerful means to functionally analyze your protein of interest without the need for an antibody specific to each new protein under study. The pCMV-Tag vectors have a single copy of the available epitope tags, while the pCMV-3Tag vectors have three copies of the epitope tag (in a variety of configurations) to prove a stronger signal in immmunological assays.
Method completed in less than one day versus 2 days with restriction enzymes
The pSG5 Vector is a 4.1 Kb eukaryotic expression vector constructed by combining pKCR2 and the Stratagene pBS vector. The high copy number of this plasmid allows for large quantities of double-stranded DNA to be obtained. Expression in vivo is achieved via transient expression in a variety of cell lines (highest level of expression is obtained following transfection of a cell line expressing the T antigen).
StrataClean resin eliminates the need to perform phenol:chloroform extractions, which are highly toxic and combustible. The resin is a non-toxic, non-combustible slurry of hydroxylated silica particles that is used to clean up DNA or concentrate dilute protein mixtures.
The StrataClone Mammalian Expression Vectors were designed for high-level expression of epitope-tagged proteins in mammalian cells. Use of StrataClone technology for the cloning steps allows rapid cloning of blunt-ended PCR products, produced using high-fidelity PCR enzymes, into the epitope tagging/expression vectors.
