Cell Death / Health Assays
ApplicationThe immunoreactivity of the M30 antibody is confined to the cytoplasm of apoptotic cells. It can be easily used for: Immunocytochemistry Flow Cytometry Immunohistochemistry: paraffin-embedded tissue sections (secondary enhancer reagents needed for this particular application) and cryostat sections Benefits Determine caspase activity, even in formalin-embedded tissue. Identify the stage of apoptosis (…
ApplicationSamples suitable for the Caspase 3 Activity Assay are: Lysates of apoptosis-induced cells Recombinant caspase 3 (CPP32) The Caspase 3 Activity Assay can be used to: Detect and quantify in vitro caspase 3 activity in cell lysates Analyze caspase 1, 2, 3, or 7 activity by simply omitting the capture-antibody step Perform inhibitor studies for Caspase 3 This kit is intended as a tool for life science…
ApplicationDuring apoptosis, the activation of specific proteases, called Caspases (cysteinyl-aspartic-acid-proteases), is one of the initial intracellular biochemical events. The Homogeneous Caspases Assay can be used to detect caspase activity in life science research applications: for sensitive quantification of activated caspases for screening of caspase inhibitors as an automatable one-step assay for high-throughp…
In 2008 Gentronix launches a new S9 protocol to extend the application of the GreenScreen HC assay to include the detection of genotoxic metabolites. Many pharmaceuticals only present a genotoxicity hazard after metabolism and such chemicals are called pro-mutagens or pro-genotoxins. Generally, although not exclusively, the liver is considered responsible for the “metabolic activation” (MA) of promutagens. However, none of t…
ATP luminescence assay for quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells. The 1step luciferase ATP assay format only requires one addition step, and can be used for continuous processing.
OpenArray® Pathways are ready-to-use gene panels containing pre-validated real-time qPCR assays. The plates have over 600 assays each and are compatible with multiple sample types. Panels are currently avaliable with human or mouse genes. New panels are continually under development. Check back for updates. Real-Time qPCR assays for Apoptosis and related genes reformatted onto an OpenArray plate. Kit includes: OpenArray plate,…
R&D Stem Cell Kits and Panels Research in developmental biology has led to the discovery of several types of stem cells, including embryonic stem (ES) cells, embryonic germ cells, and adult stem cells. Techniques and reagents developed for the in vitro culture, manipulation and characterization of stem cells provide unprecedented opportunities to study embryology and develop disease treatments. R&D Systems offers the follow…
R&D Flow Cytometry Kit - Flow cytometry kits designed for analysis of cell populations use up to four fluorochrome-conjugated antibodies. Flow cytometry facilitates quantitative, multiparameter analysis of cell populations based on the expression of cell surface and/or intracellular molecules. R&D Systems offers a wide range of biotin-, fluorescein-, phycoerythrin-, or allophycocyanin-conjugated monoclonal antibodies specific…
Guava Mitochondrial Depolarization Assay for detection of early apoptosis. There is growing interest within the scientific community to evaluate changes in the mitochondrial membrane potential and further define its role in initiating apoptosis, cell cycle, and other cellular processes. Automated single-cell analysis is the technique of choice due to its sensitivity and high reproducibility. Guava Technologies has optimized an…
