Western-Star™ Immunodetection System
Thermo Fisher ScientificThe Western-Star™ system is a versatile, non-isotopic chemiluminescent immunodetection systems.
Western Blotting Products & Reviews
Western blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Selected Filters:
I-Block™ Protein-Based Blocking Reagent
Thermo Fisher ScientificI-Block™ reagent is a highly purified casein-based blocking reagent.
Synthetic Blocking Buffer - Blotting
The protein-free Blocking Buffer is a ready-to-use solution intended for blocking of the remaining free binding sites after membrane coating. The blocking is highly effective and stable.Our Synthetic Blocking Buffer™ eliminates the risk of false positive cross reactions in the assay. Current safety hazards related to the use of bovine material, for instance BSA are avoided. The buffer is ready-to-use and offers a high level of…
OPD Tablets - 2 mg - Fizzing - Buffer-containing
OPD, Ortho-Phenylenediamine, is used as a chromogen substrate in ELISA procedures with an absorption maximum at 492 nm and produces a yellow colour during the enzymatic degradation of hydrogen peroxide by horseradish peroxidase. After addition of sulfuric acid you will obtain a very stable end solution. The fizzing, buffer containing tablets are dissolved in distilled water within few minutes. This will give solution of pH 5.2…
OPD Tablets - Fizzing
OPD, Ortho-Phenylenediamine, is used as a chromogen substrate in ELISA procedures with an absorption maximum at 492 nm and produces a yellow colour during the enzymatic degradation of hydrogen peroxide by horseradish peroxidase. After addition of sulfuric acid you will obtain a very stable end solution. The fizzing, buffer containing tablets are dissolved in distilled water within few minutes. This will give solution of pH 5.2…
OPD Tablets
OPD is used as a chromogen substrate in ELISA procedures with an absorption maximum at 492 nm and produces a yellow colour during the enzymatic degradation of hydrogen peroxide by horseradish peroxidase. After addition of sulfuric acid you will obtain a very stable end solution. Stability life time is 18 months at 4°C. Our standard tablets are available with a content of 2 mg or 10 mg OPD.
AEC Tablets - Fizzing - Buffer-containing, pH 5.1
AEC (3-amino-9-ethylcarbazole) is used as a chromogen substrate in immunoblotting and immunohistochemistry. AEC produces a rose-red colour during the enzymatic degradation of H2SO4 by horseradish peroxidase. Each tablet contains 5 mg of AEC and dissolves in approx. 5 minutes. 1 tablet is added 1 ml of 96 % alcohol, followed by addition of distilled water to 10 ml. This will give a solution of pH 5.1 + 0.1 buffered with 20 m…
DAB Tablets - Fizzing
DAB (3,3-diaminobenzidine tetrahydrochloride), is used as a chromogen substrate in immunoblotting.DAB produces a brown colour during the enzymatic degradation of H2SO4 by horseradish peroxidase. Each tablet contains 10 mg of DAB and dissolves in distilled water in less than 5 minutes. Stability life time is 18 months at 4°C.
FAST RED Tablets
Fast Red is used as a chromogen substrate in immunoblotting and immunohistochemistry using alkaline phosphatase antibody conjugates. Each tablet contains 0.5 mg naphthol phosphate, 2 mg FAST RED chromogen and 0.4 mg levamisole, an inhibitor of endogenous enzyme activity. Stability life time is 18 months at 4°C.
pNPP Substrate
The pNPP (p-NitroPhenyl Phosphate) liquid, ready-to-use substrate is a highly active and stable formulation utilized for measuring AP- (Alkaline Phosphatase) probe activity. The solution is a solvent free buffer solution containing stabilized pNPP using sodium azide as preservative. In kinetic assays p-nitrophenol appears yellow and is measured at 405 /650 nm. If stopped reactions are preferred, add equal amounts of 1 .0 M s…