PCR and Thermal Cycling Products & Reviews

The TaqPlus Precision polymerase mixture is an optimized blend of Stratagene cloned Pfu DNA polymerase and Taq2000 DNA polymerase, a highly purified, recombinant version of Taq DNA polymerase. The TaqPlus Precision PCR system exhibits the highest replication accuracy achieved using DNA polymerase mixtures.

Easy-A Hi-Fi Cloning Enzyme & Master Mix combines the higher accuracy of a proofreading enzyme with the high cloning efficiency of Taq. PCR products amplified with the Easy-A can be cloned directly, without performing additional steps typically required when amplifying with proofreading polymerases.

Discover AccuScript high-fidelity reverse transcriptase (RT). AccuScript delivers 3.7X more accuracy than other commercially available RTs and 6.6X more accuracy than AMV RT. The AccuScript PfuUltra II RT-PCR kit provides a complete system in applications that demand high fidelity, specificity, and throughput.

The AffinityScript qPCR cDNA Synthesis Kit offers efficient conversion of RNA to cDNA. Fully optimized for real-time quantitative PCR (qPCR) applications, the kit utilizes our qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.

The AffinityScript qPCR cDNA Synthesis Kit offers efficient conversion of RNA to cDNA. Fully optimized for real-time quantitative PCR (qPCR) applications, the kit utilizes our qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.

The Just cDNA Double-Stranded cDNA Synthesis Kit generates high-quality, blunt-ended cDNA, perfect for use in non-directional cDNA libraries.  Makes blunt double-stranded cDNA  Can be used for in vitro transcription  Perfect for nondirectional cDNA libraries

QuikHyb hybridization solution was developed for use in Southern, Northern, slot-blot and plaque-lift formats using randomly labeled radioactive and nonradioactive nucleic acid probes.  Reduces hybridization time from customary 12-24 hours to 1-2 hours  Efficient and sensitive radioactive and nonradioactive detection of nucleic acids  Doubles signal intensity

Taq Extender PCR additive improves the reliability and yield of conventional Taq-based PCR amplifications. Taq Extender additive increases the efficiency of Taq DNA polymerase extension reactions during each cycle of PCR by reducing mismatch pausing, resulting in a greater percentage of completed extension reactions.

The PCR grade deoxynucleotide mix includes 25 mM of each standard dNTP (GATC) for use with any PCR application, including real-time quantitative PCR. These reactions buffers contain the optimal magnesium concentration for efficient PCR.

The Universal Human miRNA Reference RNA is an ideal reference control for miRNA microarray or miRNA-targeted QRTPCR experiments. The Agilent miRNA Kit may also be used as an optimization or standardization reagent for human miRNA analysis. Available in industrial lots for high throughout applications.

Identify fish species quickly, easily, and accurately. Whether your samples are fresh, frozen, dried, salted, or minced, Agilent’s DNA Fish Species Identification solution provides reliable, robust answers within hours. And, the method is far more accurate than looking at a fish and guessing.

The Agilent SARS-CoV-2 qRT-PCR Dx kit is a real-time reverse transcriptase PCR (qRT-PCR) in vitro diagnostic test for qualitative detection of SARS-CoV-2 RNA.

Reveal the molecular signatures of tumor immune responses

Double-quenched probes that offer improved quenching over longer probe sequences

Combining throughput, efficiency and robust design, the Hydrocycler range of water bath thermal cyclers offer an attractive alternative to traditional Peltier-based thermal cyclers for high-throughput PCR.

Formulated with diagnostic kit developers in mind, RapiDxFire™ qPCR 5X Master Mix glycerol free (GF) can be combined with gene-specific primers and hydrolysis probes for immediate use in high-throughput laboratory developed test (LDT) workflows or further processed for lyophilisation for use in point of care (POC) devices. Provided at a 5X concentration without passive reference dye, this flexible master mix allows more room…

The Soellex™ is an ultra-high-throughput end-point PCR thermal cycler leveraging a 3-chamber water bath.

The Yale 2019-nCoV SalivaDirect™ ValuPanel Reagents™ include FAM-BHQ and Quasar™ 670-BHQ probes and primers delivered in individual tubes in dried format.

Choose premier KASP on Demand (KOD) service for design and delivery of your KASP Assay, plus full validation and optimization of your assay by our scientific team.  

Designed with input from top molecular diagnostic assay manufacturers, the RapiDxFire Lyo-Flex 1-Step RT-qPCR 5x Master Mix supports the development of diagnostic devices and assays that meet healthcare provider and consumer demand for fast, accurate results. With consistent results in a liquid state at room temp or in a lyophilised state, this master mix will help you achieve predictable performance in unpredictable conditio…

You know what you’re looking for and we’re here to help you find it. Our highly qualified molecular biologists and PCR assay design experts act as an extension of your team, designing the best qPCR assay for your target of interest and experimental conditions.  

CPG used to add the non-fluorescent quencher BHQ-1 dye to the 3' end of an oligonucleotide.

Ribonuclease R (RNase R) from E. coli is a magnesium-dependent 3ʹ to 5ʹ exoribonuclease available for circular RNA cleanup, alternative splicing research, and gene expression studies.  Key features:  Efficacy and quality assured under ISO 13485 certification Available in bulk and for custom configurations Effectively degrades linear RNA, Y-structure RNAs, but not lariat loops or circular RNAs Digests linear RNAs…

Expand your in-house food safety PCR testing capabilities with a high-performance benchtop platform: Carry out rapid food protection testing with the Applied Biosystems™ QuantStudio™ 5 Food Safety Real-Time PCR System. The affordable and easy-to-use system is ideal for users who need superior performance, maximum dye versatility, and security options in a real time PCR system.

Rapidly detect and differentiate Salmonella species, Salmonella ser. Typhimurium, and Salmonella ser. Enteritidis in a single test: The Thermo Scientific™ SureTect™ Salmonella Species, Typhimurium and Enteritidis Multiplex PCR Assay enables you to rapidly identify and distinguish key Salmonella strains of concern in a single test.The workflows for testing poultry and pork meats, as well as production environment samples, ha…