PCR and Thermal Cycling Products & Reviews

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Polymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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PAQ5000 2X PCR Master Mix

Agilent Technologies

The Paq5000 DNA polymerase is a robust Taq substitute that is derived from a Pyrococcus species. This enzyme provides equivalent and often better performance to endpoint Taq DNA polymerase amplification and can be substituted easily into many Taq-based procedures.  Robust, cost-effective alternative to Taq  Equal to better yield with faster cycling conditions  Amplify up to 6 kb genomic DNA targets  Easily substituted…

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TaqPlus Precision PCR System

Agilent Technologies

The TaqPlus Precision polymerase mixture is an optimized blend of Stratagene cloned Pfu DNA polymerase and Taq2000 DNA polymerase, a highly purified, recombinant version of Taq DNA polymerase. The TaqPlus Precision PCR system exhibits the highest replication accuracy achieved using DNA polymerase mixtures.

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Easy-A High-Fidelity PCR Master Mix

Agilent Technologies

Easy-A Hi-Fi Cloning Enzyme & Master Mix combines the higher accuracy of a proofreading enzyme with the high cloning efficiency of Taq. PCR products amplified with the Easy-A can be cloned directly, without performing additional steps typically required when amplifying with proofreading polymerases.

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QuikHyb Rapid Hybridization Solution

Agilent Technologies

QuikHyb hybridization solution was developed for use in Southern, Northern, slot-blot and plaque-lift formats using randomly labeled radioactive and nonradioactive nucleic acid probes.  Reduces hybridization time from customary 12-24 hours to 1-2 hours  Efficient and sensitive radioactive and nonradioactive detection of nucleic acids  Doubles signal intensity

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Taq Extender PCR Additive

Agilent Technologies

Taq Extender PCR additive improves the reliability and yield of conventional Taq-based PCR amplifications. Taq Extender additive increases the efficiency of Taq DNA polymerase extension reactions during each cycle of PCR by reducing mismatch pausing, resulting in a greater percentage of completed extension reactions.

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Universal miRNA Reference Kit

Agilent Technologies

The Universal Human miRNA Reference RNA is an ideal reference control for miRNA microarray or miRNA-targeted QRTPCR experiments. The Agilent miRNA Kit may also be used as an optimization or standardization reagent for human miRNA analysis. Available in industrial lots for high throughout applications.

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PCR-RFLP Fish ID Kit

Agilent Technologies

Identify fish species quickly, easily, and accurately. Whether your samples are fresh, frozen, dried, salted, or minced, Agilent’s DNA Fish Species Identification solution provides reliable, robust answers within hours. And, the method is far more accurate than looking at a fish and guessing.

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ARM-D® Kit, ampC

Streck

The Streck ARM-D kit, ampC is a multiplex real-time PCR kit for the rapid detection of AmpC β-lactamase resistance genes in Gram-negative bacteria. This comprehensive kit allows you to detect over 200 ampC allelic variants in the clinically relevant resistance gene families, CMY02, DHA, ACC, EBC, FOX and MOX, and differentiate between plasmid-mediated and chromosomal AmpC β-lactamase resistance. 

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ARM-D® Kit, TEM/SHV/GES

Streck

The Streck ARM-D Kit, TEM/SHV/GES is a multiplex real-time PCR kit for the rapid detection of antibiotic resistance genes in Gram-negative bacteria. This comprehensive kit allows you to detect over 350 allelic variants in the TEM, SHV and GES extended-spectrum β-Lactamase gene families. 

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Biometra TRIO Thermal Cycler – Triple powered PCR

Analytik Jena GmbH+Co. KG

Threefold power for maximum flexibility in PCR. The thermal cycler Biometra TRIO provides three independent sample blocks which can run three different PCR reactions in parallel. The instrument is available with different sample blocks: 3x48 well for 0.2 ml tubes, 3x30 well for 0.5 ml tubes or 3x combi block for 48 well 0.2 ml tubes or 18x0.5 ml tubes each. Thus, this thermocycler offers high throughput in parallel operatio…

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qTOWER iris: The Real-Time PCR-Thermocycler

Analytik Jena GmbH+Co. KG

No matter where your journey takes you in the world of qPCR, this real-time PCR thermal cycler is ready for anything, giving you full control and freedom of choice - from consumables to assays to colour modules. The instrument is operating quietly, smoothly and delivers reliable results on six channels. The temperature and readout accuracy are unsurpassed. With this open system you are well equipped for all your projects.

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BRAND PCR Tubes, Strips, and Plates

BRANDTECH® Scientific, Inc.

All BRAND PCR products are available in white plastic, uniformly colored with titanium dioxide (TiO2) for optimal results when performing quantitative PCR (gPCR) with fluorescent signals.   BRAND microcentrifuge tubes fit most common rotors and may be spun up to 20,000 RCF (10,000 RCF for 0.5mL). Manufactured from virgin polypropylene, these highly translucent tubes are free from flash, air bubbles, and streaks. Caps are…

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