Cell Based Assays
The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated Histone H2A.X. Cells are cultured in microplates, treated with agents that induce DNA damage or apoptosis, which stimulates H2A.X phosphorylation. Cells are then fixed and permeabilized in preparation for staining and detection. Histone H2A.X phosphorylated at serine 139 is detected by…
TOPglow (TCF reporter plasmid): Transfection grade T cell factor (TCF) reporter plasmid containing four copies of the Tcf binding site (wt) upstream of E1B TATA box and Luciferase open reading frame.
The ApopTag ISOL Fluorescence Apoptosis Detection (DNase Types I & II) technique is based upon the biochemical specificity of the enzymes T4 DNA ligase and Vaccinia Virus DNA Topoisomerase I and the unique, dual labeled, dual hairpin oligonucleotide
The CHEMICON APO-DIRECT™ Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry (Li et al. 1995). The kit contains all the reagents required for measuring apoptosis in cells including positive and negative control cells; washing, reaction, and rinsing buffers; and propidium iodide/RNase A solution for counter staining the total DNA.
The MitoLight™ Apoptosis Detection Kit utilizes a lipophilic cation, termed as MitoLight™, as a mitochondrial activity marker. MitoLight™ is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. The monomer is in equilibrium with so-called J-aggregates, which are favored at higher dye concentration or higher mitochondrial membrane potential. MitoLight™ partitions differently i…
The CHEMICON In Situ FLICA Pan-Caspase Detection Kit is a fluorescent-based assay for detection of active caspases in cells undergoing apoptosis. The kit is for research use only. Not for use in diagnostic or therapeutic procedures.
The CHEMICON In Situ FLICA Caspase-3/7 Detection Kit is a fluorescent-based assay for detection of active caspase-3 or caspase-7 in cells undergoing apoptosis. The kit is for research use only. Not for use in diagnostic or therapeutic procedures.
The CHEMICON In Situ FLICA Caspase-3 Detection Kit is a fluorescent-based assay for detection of active caspase-3 in cells undergoing apoptosis. The kit is for research use only. Not for use in diagnostic or therapeutic procedures.
AXIS devices physically isolate developing neurites from each other and their respective neural cell bodies. In addition, the use of hydrostatic pressure during analysis provides researchers with an opportunity for targeted exposure of neuronal parts to growth factors, drug compounds, or other reagents of biological interest. This ability to selectively control exposure of neuronal outgrowth makes AXIS a powerful platform for…
Collagen-induced arthritis (CIA) in mice is widely used as an experimental model for rheumatoid arthritis (RA) in humans. CIA is mediated by autoantibodies, which bind to a particular region of type II collagen, such as CB11 (CNBR-digested fragment of type II collagen) (Terato et al, 1985). Antibody-mediated CIA can be induced by i.v. injection of a combination of four different monoclonal antibodies generated by the ArthroMAB…
