Polymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
The Universal Human miRNA Reference RNA is an ideal reference control for miRNA microarray or miRNA-targeted QRTPCR experiments. The Agilent miRNA Kit may also be used as an optimization or standardization reagent for human miRNA analysis. Available in industrial lots for high throughout applications.
Identify fish species quickly, easily, and accurately. Whether your samples are fresh, frozen, dried, salted, or minced, Agilent’s DNA Fish Species Identification solution provides reliable, robust answers within hours. And, the method is far more accurate than looking at a fish and guessing.
With a uniform thermal ramp rate of over 10°C per second xxpress™ is the fastest real-time thermocycler in the World delivering 40 cycle q-PCR in under 10 minutes. And whilst not all PCR chemistries are yet as fast as this system there are a number that repeatably and reliably work at this speed. Ultra low thermal mass and high conductively enable the patented resistive heating system deliver this speed cycle more efficiently…
An arrayed or non-arrayed BAC library is the preferred choice for downstream applications such as hybridization screening; full-genome sequencing; BAC end sequencing or physical mapping projects. Amount of clones received is based on a guaranteed coverage and 120Kb average insert size. HMW DNA isolation and QC by HMW DNA enzymatic digestion are included. Deliverables for ARRAYED BAC library: Clones will be arrayed and amplifie…
A pooled BAC library is an invaluable tool for PCR-screening. It allows for the easy retrieval of BAC clones of interest. This type of library is especially useful when genome size is large and creating an arrayed library is cost-prohibitive. We typically construct a minimum 5X BAC library and BAC clones are then pooled and distributed in 96-well plates. Each well will contain about 400-500 original BAC clones. HMW DNA isolati…
Our uncloned normalized cDNA is created using proprietary technology which features full-length-enriched and high quality size-fractionated cDNA. Our cDNA has been used successfully with FLX, Titanium an Illumina sequencing.
Our cDNA libraries feature full-length-enriched and high quality size-fractionated cDNA. cDNA library construction is usually done in slightly-modified versions of pBluescript or pcDNA3.1. Insert sizes will range from about 0.5Kb to 4Kb. Concerning QC steps, we evaluate the average insert size, empty clone rate and clone concentration/tube before shipping out. We always provide a gel picture of randomly selected clones showing…
ASH is APA-based, full-length-enriched, single-stranded cDNA subtraction hybridization library. - ASH cDNA subtraction is full-length-enriched and independent of PCR suppression. - ASH cloning is directional and does not involve normalization. - ASH method provides non-exponential amplification before subtraction because double-stranded cDNA is not required. - ASH method has a better subtraction effect because 1) a high ratio…
RealTime ready Custom RT-qPCR Assays and Panels Get a head start on your gene expression analysis with function tested assays for all annotated human, mouse, and rat genes Achieve high specificity using intron-spanning assays based on short hydrolysis LNA-substituted Universal ProbeLibrary (UPL) Probes. Rely on stringently RT-qPCR-tested assays that are compliant with MIQE (Minimum Information for Publication of Quantitati…
A Billion Reactions. Digital Answers. Experience the Next Generation of PCR. Capable of generating more than a billion reactions in a single day, the RainDrop™ Digital PCR System surpasses all existing technologies and establishes a new performance standard in sensitivity, quantitation, and multiplexing.Based on RainDance’s proven picodroplet platform, the RainDrop System delivers absolute quantitation of target molecules. Th…
Enclosed – safe – sound: Xiril’s Neon100 with Hood Xiril liquid handling robots are ideally suited to perform a large number of liquid handling tasks in automation solutions. E.g. processes such as nucleic acid separation or PCR setup can easily be automated. The proven pipetting robot Neon is available with up to 36 positions on the worktable, carrying one or two robotic arms facilitating your daily pipetting routine. The bas…
A 38µm soft non-permeable aluminum foil sealing film with strong medical grade adhesive, AlumaSeal II™ sealing films eliminate the need for heat-sealing devices or mats during thermal cycling. Compared to other aluminum foils, AlumaSeal II™ has less tendency to roll back on itself when removing the backing paper and conforms well to the plate during applications. • Easily pierceable with single or multi-channel pipettors and…
For DMPK sample card punching and pipetting. The easyPunch STARlet consists of a STARlet Autoload liquid handling workstation comprising 4 ndependent 1 ml channels, a card and plate gripper and the punch module. computer with Hamilton enus software and 4 specific predefined methods is included as well.The system can be used for a variety of different applications e. g. forensic reference databasing, MPK and toxicology studie…
Made of thin wall polypropylene and designed to fit on all Corning® Thermowell™ GOLD 1 x 8 well PCR strips and 96 well PCR microplates DNase- and RNase-free Autoclavable at 121°C
Thermo Scientific* Fermentas PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs and all the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR. ■ Convenient—Taq DNA Polymerase in a ready-to-use mix ■ Fast—sa…
The MagNA Pure LC Cooling Block, LC Centrifuge Adapters, holds 32 LightCycler® Centrifuge Adapters containing LightCycler® Capillaries (20 µl). The Cooling Block is designed to cool the capillaries during automated PCR setup by the MagNA Pure LC Instrument. In addition, the Cooling Block holds fifteen 1.5 ml reaction tubes (e.g., for master mixes). The stage of the MagNA Pure LC Instrument includes a cooling area, which is des…
The MagNA Pure LC Cooling Block, LC Sample Carousel holds one LightCycler® Sample Carousel (all formats) containing LightCycler® Capillaries. The Cooling Block is designed to cool the capillaries during automated PCR setup by the MagNA Pure LC Instrument. In addition, the Cooling Block holds sixteen 1.5 ml reaction tubes (e.g. for master mixes). The stage of the MagNA Pure LC Instrument includes a cooling area, which is design…
Application The MagNA Pure LC Cooling Block, Reaction Tubes, is used for placing thirty-two 1.5 ml reaction tubes, with screw caps, on the stage of the MagNA Pure LC Instrument. This Cooling Block can be combined with either the MagNA Pure LC Cooling Block, LC Centrifuge Adapters, or 96-well PCR Plate. The stage of the MagNA Pure LC Instrument includes a cooling area that is designed for various post-elution pipetting proces…
Application The MagNA Pure LC Cooling Block, A-Ring holds one or two A-Rings with bar codes. Application of the corresponding Post Elution Module of the MagNA Pure LC Software results in automatic filling of the A-Rings with purified nucleic acids and compatible PCR reagents, which are also positioned on the Cooling Block. The A-Rings are then ready to be transferred to an instrument platform for testing. The stage of th…
Easy-to-use, one-component reagent for internal labeling of oligonucleotide probes to be detected on the LightCycler® and LightTyper Instruments. Application The SimpleProbe 519 Labeling Reagent is used for the internal labeling of oligonucleotide probes that are to be detected at 519 nm, for example, when used as mutation detection probes on the LightCycler® and LightTyper Systems following the SimpleProbe probe principle.…
GeneAmp® PCR System 9700 is specifically designed for the amplification of nucleic acids. This system consists of a base module and one of many interchangeable sample block modules. The Auto-Lid Dual 384-well GeneAmp® PCR System 9700 is designed for use with 384-well reaction plates for high-throughput PCR and cycle sequencing applications. Automated heated lid delivers precision movement with a 15-second opening and closing t…
GeneAmp® PCR System 9700 is specifically designed for the amplification of nucleic acids. The GeneAmp® PCR System 9700 consists of a base module and one of many interchangeable sample block modules. The Auto-Lid Dual 384-well GeneAmp® PCR System 9700 is designed for use with 384-well reaction plates for high-throughput PCR and cycle sequencing applications. • Compact design to maximize bench space • Two 384-well sample blocks…
LightCycler® Nano Instrument - Real-time PCR amplification and detection instrument for tube-based assays of up to 32 (4 x 8) samples per run, with minimal noise, footprint and weight. Assembled from just a few building blocks, the robust LightCycler® Nano System is easy to transport and install. Up to 32 samples can be run in 0.2 ml tubes connected as strips. Fast heating and cooling is done using Peltier elements. Assay per…
The Thermo Scientific* SureTect Listeria monocytogenes PCR Assay is a multiplex real-time assay that incorporates unique primer and probe components for highly-specific detection of the prfA gene, specific to and found only in Listeria monocytogene.The assay also includes primer and probe components, for simultaneous detection of an internal amplification control (IAC), for confirmation of successful PCR and increased confiden…
