ELISA Reagents and Buffers Products & Reviews

Discover our breadth of experience and be reassured that our Lateral Flow  Development Services will deliver the best outcome for your assay.

BBI Solutions has over 25 years’ expertise working with customers and partners to develop and manufacture high quality proteins for a diverse range of applications with our Custom Protein Service.

BBI’s custom conjugation service allows us to develop high-quality conjugates using reagents provided by you or sourced by us.

BBI Solutions’ Novarum™ technology transforms a smartphone into a mobile diagnostic platform, empowering users to read and share the results of rapid tests at the point of care.  

ApplicationThe kit is used for the quantitative determination of BrdU incorporated into cellular DNA using a 96-well microplate cell ELISA format. Benefits Safer: No radioisotopes are used. Accurate: The results strongly correlate to those obtained with the [3H]-thymidine method. Sensitive: As sensitive as [3H]-thymidine. Fast: The use of multiwell ELISA readers allows for processing of a large number of samples. Ea…

The Alkaline Phosphatase is purified from calf intestine by a method developed in our laboratories, involving affinity chromatography. This enzyme is widely used as a marker enzyme in applications such as: • ELISA Immunoblotting Immunohistochemistry Molecular Biology Activity: >630 U*/mg enzyme protein (25°C, 4-nitrophenyl phosphate, glycine as buffer) • Unit definition: That amount of enzyme causing the hydrolysis of one mi…

The WellChampion™ preserves and protects biological activity of proteins and other biomolecules coated and dried on microwell surfaces. The protection of the coated and dried surface prevents degradation, denaturation and leaching. The WellChampion™ also efficiently blocks any free binding sites left on the microwell surface after coating, and a separate blocking step is avoided. Simply coat the microwell plate with the biomol…

The protein-free Blocking Buffer is a ready-to-use solution intended for blocking of the remaining free binding sites after microplate coating. The blocking is highly effective and stable. Our Synthetic Blocking Buffer™ eliminates the risk of false positive cross reactions in the assay. Current safety hazards related to the use of bovine material, for instance BSA are avoided. The buffer is ready-to-use and offers a high level…

OPD, Ortho-Phenylenediamine, is used as a chromogen substrate in ELISA procedures with an absorption maximum at 492 nm and produces a yellow colour during the enzymatic degradation of hydrogen peroxide by horseradish peroxidase. After addition of sulfuric acid you will obtain a very stable end solution. The fizzing, buffer containing tablets are dissolved in distilled water within few minutes. This will give solution of pH 5.2…

OPD, Ortho-Phenylenediamine, is used as a chromogen substrate in ELISA procedures with an absorption maximum at 492 nm and produces a yellow colour during the enzymatic degradation of hydrogen peroxide by horseradish peroxidase. After addition of sulfuric acid you will obtain a very stable end solution. The fizzing, buffer containing tablets are dissolved in distilled water within few minutes. This will give solution of pH 5.2…

OPD is used as a chromogen substrate in ELISA procedures with an absorption maximum at 492 nm and produces a yellow colour during the enzymatic degradation of hydrogen peroxide by horseradish peroxidase. After addition of sulfuric acid you will obtain a very stable end solution. Stability life time is 18 months at 4°C. Our standard tablets are available with a content of 2 mg or 10 mg OPD.

DAB (3,3-diaminobenzidine tetrahydrochloride), is used as a chromogen substrate in immunoblotting.DAB produces a brown colour during the enzymatic degradation of H2SO4 by horseradish peroxidase. Each tablet contains 10 mg of DAB and dissolves in distilled water in less than 5 minutes. Stability life time is 18 months at 4°C.

Fast Red is used as a chromogen substrate in immunoblotting and immunohistochemistry using alkaline phosphatase antibody conjugates. Each tablet contains 0.5 mg naphthol phosphate, 2 mg FAST RED chromogen and 0.4 mg levamisole, an inhibitor of endogenous enzyme activity. Stability life time is 18 months at 4°C.

The pNPP (p-NitroPhenyl Phosphate) liquid, ready-to-use substrate is a highly active and stable formulation utilized for measuring AP- (Alkaline Phosphatase) probe activity. The solution is a solvent free buffer solution containing stabilized pNPP using sodium azide as preservative. In kinetic assays p-nitrophenol appears yellow and is measured at 405 /650 nm. If stopped reactions are preferred, add equal amounts of 1 .0 M s…

The liquid BCIP/NBT is the most commonly used substrate for colorimetric determination of Alkaline Phosphatase (AP) activity in membrane tests. We offer an aqueous buffered solution containing NBT (NitroBlue Tetrazolium) and BCIP (Bromo-Chloro-Indolyl Phosphate) forming a blue/purple precipitate at the positive sites. The colour develops when AP catalyses the dephosphorylation of BCIP. The intense blue/purple precipitate is…

TMB (3,3´,5,5´- tetramethylbenzidine) is a substrate for horseradish peroxidase (HRP). Our mono-component, liquid TMB solutions combine TMB, substrate buffer and hydrogen peroxide for a safe, ready-to-use, one step, chromogen system. The products do not contain harmful solvents. Recommended for ELISA procedures, liquid TMB yields a soluble blue end product read at 655 nm. The TMB reaction may be stopped with 0.2 M H2SO4 resu…

This new type of TMB with a red indicator combines all the important qualities of TMB ONE, eliminating possible mistakes during pipetting. Further the red colour may be useful in automatic analyzers giving the warning "Reagent low" when it is time for filling up the substrate.The red indicator disappears after addition of the stopping solutionand will neither affect the sensitivity nor the stability of the substrate. Stability…

The TMB PLUS has a higher activity level, resulting in a higher sensitivity and a reduced incubation time than found using the TMB ONE. TMB is a substrate for HRP. The mono-component, liquid TMB solution combines TMB, substrate buffer and hydrogen peroxide for a safe, ready-to-use, one step, chromogen system.The product does not contain harmful solvents. Recommended for ELISA procedures, liquid TMB yields a soluble blue end pr…

This new type of TMB with a red indicator combines all the important qualities of TMB PLUS, eliminating possible mistakes during pipetting.Further the red colour may be useful in automatic analyzers giving the warning "Reagent low" when it is time for filling up the substrate. The red indicator disappears after addition of the stopping solution and will neither affect the sensitivity nor the stability of the substrate. Stabili…

TMB is a substrate for HRP. Our mono-component, liquid TMB solution combine TMB, substrate buffer and hydrogen peroxide for a safe, ready-to-use, one step, chromogen system.The products do not contain harmful solvents. The TMB X-tra is a unique TMB substrate with an excellent resistancy and the highest sensitivity.

This TMB X-tra with red indicator combines all the important qualities of TMB X-tra eliminating possible mistakes during pipetting.Further the red colour may be useful in automatic analyzers giving the warning “Reagent low” when it is time for filling up the substrate. The red indicator disappears after addition of the stopping solution and will neither affect the sensitivity nor the stability of the substrate.

10 OD to 50 OD concentrated nanoparticles that have been optimized for rapid assays without the traditional boiling and centrifugation steps. The BioAssay Works gold sol production process is unique, by eliminating the boiling step, and because of the concentrated gold, you do not have to dilute the sols prior to conjugation, nor do they have to be centrifuged to remove free antibody. The elimination of these two steps alone i…

The Au/Ag particles have increased advantages in light or laser applications, when taking light scattering measurements.  The BioAssay Works silver/gold nanoparticles and conjugates production process is unique, by eliminating the boiling step, and because of the concentrated gold, you do not have to dilute the sols prior to conjugation, nor do they have to be centrifuged to remove free antibody. The elimination of these two s…

New pNPP ONE Substrate – The pNPP (p-NitroPhenyl Phosphate) liquid, ready-to-use substrate is a highly active and stable formulation utilized for measuring AP- (Alkaline Phosphatase) probe activity. The solution is a solvent free buffer solution containing stabilized pNPP using sodium azide as preservative. In kinetic assays p-nitrophenol appears yellow and is measured at 405 /650 nm. If stopped reactions are preferred, add…

Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…