Kits
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's Caspase-8 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate IETD-pNA. The free pNA can be quantified using a s…
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's Caspase-9 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the LEHD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate LEHD-pNA. The free pNA can be quantified using a spectropho…
The CHEMICON Glutathione Detection Kit is a quick method for detection of intracellular levels of reduced glutathione (GSH). Reduction in levels of GSH may be an early indicator of apoptosis.
The CHEMICON Proteasome Activity Assay Kit provides a quick, efficient and sensitive system for evaluation of proteasome activity in cell lysates or inhibitor screening. Chemicon's Proteasome Activity Assay Kit provides a simple and convenient means for assaying proteasome activity that recognize the substrate LLVY (Meng et al. 1999). The assay is based on detection of the fluorophore 7-Amino-4-methylcoumarin (AMC) after cle…
MTT is a pale yellow substrate that is cleaved by living cells to yield a dark blue formazan product. This process requires active mitochondria, and even freshly dead cells do not cleave significant amounts of MTT. The colorimetric assay described below can be used for either proliferation or complement-mediated cytotoxicity assays.
Reagents and materials supplied in the Blood Vessel Staining Kit are sufficient for staining 100 tissue sections and when stored at 2-8C is stable up to the expiration date. ECM590 detects vWF and CD31 in human paraffin embedded tissues. In rat or mouse tissues only the vWF antibody is effective in paraffin; the CD31 antibody does not react with rodent CD31. CHEMICON's Blood Vessel Staining Kit is a very sensitive immunohistoc…
CHEMICON's Blood Vessel Staining Kit is a very sensitive immunohistochemical tool for the detection of vascular endothelial cells, containing both anti-von Willebrand Factor and anti-CD31 primary antibodies and is applicable to a variety of species. This AP kit provides a simple, all-in-one method for the staining of frozen and paraffin-embedded sections in human (both CD31 and vWF) or rat and mouse (vWF only) tissues
The CHEMICON uPA Activity Assay Kit provides a quick, efficient and sensitive system for evaluation of uPA activity and for screening of uPA inhibitors. The assay is colorimetric and does not require radioactivity or fluorescence equipment. The assay is sensitive over a range of 0.05-50 units of uPA activity. The CHEMICON uPA Activity Assay Kit utilizes a chromogenic substrate, which is cleaved by active uPA. Addition of this…
CHEMICON®'s Osteogenesis Quantitation Kit provides all the necessary reagents and a protocol to enable quantification of osteogenesis using a standard plate reader. This product is useful for studying the effects of growth factors, drugs and toxic agents on bone formation and for screening candidate osteogenic pharmaceuticals. Also, studies examining the intracellular signaling pathways regulating osteoblast differentiation ca…
The OxyBlot™ Protein Oxidation Detection Kit provides the chemical and immunological reagents necessary to perform the immunoblot detection of carbonyl groups introduced into proteins by oxidative reactions with ozone or oxides of nitrogen or by metal catalyzed oxidation.
OxyBlot™ Protein Standard is composed of five proteins (phosphorylase B, BSA, ovalbumin, carbonic anhydrase, trypsin inhibitor) containing 1-3 dinitrophenylhydrazone (DNP) residues per protein. These attached DNP moieties permit the visualization of the proteins by the detection system of the OxyBlot™ Protein Oxidation Detection Kit. OxyBlot™ Protein Standard serves as an internal control for the electrophoresis, western trans…
Streptavidin isolated from Streptomyces avidinii covalently bound to agarose.
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP's, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release® is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been…
The IHC Select® Immunoperoxidase Secondary Detection System is intended for use with the CHEMICON IHC Select® Prediluted Primary Antibody reagents, which contain rabbit and mouse IgG for the qualitative identification of antigens by light microscopy in paraffin-embedded tissues. The IHC Select® Immunoperoxidase Secondary Detection System may also be used with other rabbit or mouse primary antibodies. The IHC Select® Immunopero…
Our Mycoplasma Plus PCR Primer Set detects infection by all forms of Mycoplasma with a robust single-band PCR product (triplet for Acholeplasma). This kit also includes controls to verify PCR amplification and Mycoplasma genomic DNA. The primer design permits higher annealing temperatures, reducing spurious bands from PCR contaminants.
The MycoSensor PCR & QPCR Assay Kits detect Mycoplasma infection of cell cultures by real-time quantitative PCR (qPCR) or PCR in less than two hours.
The GeneMorph II Random Mutagenesis Kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis. Other methods or commercially available kits that rely on Taq DNA polymerase for random mutagenesis have shown biases towards mutating A’s and T’s more frequently than G’s and C’s, which undoubtedly skews representation of random mutant libraries.
The Agilent High Sensitivity Protein 250 kit provides protein detection in the low picogram range. The kit is ideally suited to solve typical protein QA/QC questions and to comply with ICH and FDA guidelines.
The Agilent High Sensitivity Protein 250 kit provides protein detection in the low picogram range. The kit is ideally suited to solve typical protein QA/QC questions and to comply with ICH and FDA guidelines.
The second generation of our QuikChange method that provides improved fidelity over our original kit, while maintaining greater than 80% mutation efficiency for single site mutagenesis.
The second generation of our QuikChange method that provides improved fidelity over our original kit, while maintaining greater than 80% mutation efficiency for single site mutagenesis.
The fastest and latest generation of the market-leading QuikChange kits speeds up the protocol for performing single and multiple site-directed mutagenesis to less than 3 hours.
The fastest and latest generation of the market-leading QuikChange kits speeds up the protocol for performing single and multiple site-directed mutagenesis to less than 3 hours.
The fastest and latest generation of the market-leading QuikChange kits speeds up the protocol for performing single and multiple site-directed mutagenesis to less than 3 hours.
