RNA Interference Products & Reviews

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RNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.

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PathDetect pMUT-cJun Plasmid

Agilent Technologies

The PathDetect in vivo signal transduction pathway trans-reporting systems are designed for specific, rapid, and convenient assessment of the in vivo activation of transcription activators and upstream signal transduction pathways. The systems are useful for identifying whether a newly cloned gene is involved in a signal transduction pathway, and if so, at which step of the pathway.  Identify specific stimuli that leads to th…

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PathDetect pMUT-CREB Plasmid

Agilent Technologies

The PathDetect in vivo signal transduction pathway trans-reporting systems are designed for specific, rapid, and convenient assessment of the in vivo activation of transcription activators and upstream signal transduction pathways. The systems are useful for identifying whether a newly cloned gene is involved in a signal transduction pathway, and if so, at which step of the pathway.  Identify specific stimuli that leads to th…

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PathDetect pMUT-ELK1 Plasmid

Agilent Technologies

The PathDetect in vivo signal transduction pathway trans-reporting systems are designed for specific, rapid, and convenient assessment of the in vivo activation of transcription activators and upstream signal transduction pathways. The systems are useful for identifying whether a newly cloned gene is involved in a signal transduction pathway, and if so, at which step of the pathway.  Identify specific stimuli that leads to th…

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PathDetect pNFAT-hrGFP Cis-Reporting Plasmid

Agilent Technologies

The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.  Identify pathway-specific transcription activation  Easier,…

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PathDetect pNF-kB-hrGFP Cis-Reporting Plasmid

Agilent Technologies

The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.  Identify pathway-specific transcription activation  Easier,…

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PathDetect pNFkB-Luc Plasmid

Agilent Technologies

The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.  Identify pathway-specific transcription activation  Easier,…

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PathDetect pSRE-Luc Plasmid

Agilent Technologies

The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.  Identify pathway-specific transcription activation  Easier,…

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PathDetect pSRF-Luc Plasmid

Agilent Technologies

The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.  Identify pathway-specific transcription activation  Easier,…

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PathDetect pTARE-Luc Plasmid

Agilent Technologies

The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.  Identify pathway-specific transcription activation  Easier,…

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pBC KS(-) Phagemid

Agilent Technologies

The pBC vectors were derived from the pBluescript II phagemid. The ampicillin-resistance gene has been replaced with the chloramphenicol resistance gene. pBC phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and g…

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pBC KS(+) Phagemid

Agilent Technologies

The pBC vectors were derived from the pBluescript II phagemid. The ampicillin-resistance gene has been replaced with the chloramphenicol resistance gene. pBC phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and g…

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pBC SK(-) Phagemid

Agilent Technologies

The pBC vectors were derived from the pBluescript II phagemid. The ampicillin-resistance gene has been replaced with the chloramphenicol resistance gene. pBC phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and g…

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pBC SK(+) Phagemid

Agilent Technologies

The pBC vectors were derived from the pBluescript II phagemid. The ampicillin-resistance gene has been replaced with the chloramphenicol resistance gene. pBC phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and g…

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pCAL-c Vector

Agilent Technologies

The Affinity protein expression and purification system provides a simple, gentle and effective method for high-level protein expression and purification. The complete system includes pCAL expression vectors containing a calmodulin-binding peptide tag and calmodulin affinity resin (available separately).  pCAL vectors derived from T7 RNA polymerase-based pET vectors€_€_  Novel purification tag combines small size, gentle el…

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pCAL-n Vector

Agilent Technologies

The Affinity protein expression and purification system provides a simple, gentle and effective method for high-level protein expression and purification. The complete system includes pCAL expression vectors containing a calmodulin-binding peptide tag and calmodulin affinity resin (available separately).  pCAL vectors derived from T7 RNA polymerase-based pET vectors€_€_  Novel purification tag combines small size, gentle el…

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pCAL-n-EK Vector

Agilent Technologies

The Affinity protein expression and purification system provides a simple, gentle and effective method for high-level protein expression and purification. The complete system includes pCAL expression vectors containing a calmodulin-binding peptide tag and calmodulin affinity resin (available separately).  pCAL vectors derived from T7 RNA polymerase-based pET vectors€_€_  Novel purification tag combines small size, gentle el…

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pCAL-n-FLAG Vector

Agilent Technologies

The Affinity protein expression and purification system provides a simple, gentle and effective method for high-level protein expression and purification. The complete system includes pCAL expression vectors containing a calmodulin-binding peptide tag and calmodulin affinity resin (available separately).  pCAL vectors derived from T7 RNA polymerase-based pET vectors€_€_  Novel purification tag combines small size, gentle el…

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pCFB-EGSH Retroviral Vector

Agilent Technologies

 Dose-responsive inducible mammalian expression  Induction as high as 1,700 fold  Tightly repressed  Novel IRES site allows expression of both receptor subunits from the same CMV promoter  Retroviral and plasmid gene delivery systems available  Stable receptor cell lines available

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pCMV-3Tag-2 Epitope Tagging Mammalian Expression Vector

Agilent Technologies

The pCMV-3Tag vectors are a series of epitope tagging mammalian expression vectors featuring three copies of the epitope tag in a variety of configurations. pCMV-Tag vectors have a single copy of the available epitope tags, resulting in weaker tagging.  Stronger signal in immunological assays  Small tags do not interfere with functional analysis studies  Three reading frames for both N- and C- terminus tags

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pCMV-3Tag-3 Epitope Tagging Mammalian Expression Vector

Agilent Technologies

The pCMV-3Tag vectors are a series of epitope tagging mammalian expression vectors featuring three copies of the epitope tag in a variety of configurations. pCMV-Tag vectors have a single copy of the available epitope tags, resulting in weaker tagging.  Stronger signal in immunological assays  Small tags do not interfere with functional analysis studies  Three reading frames for both N- and C- terminus tags

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pCMV-3Tag-4 Epitope Tagging Mammalian Expression Vector

Agilent Technologies

The pCMV-3Tag vectors are a series of epitope tagging mammalian expression vectors featuring three copies of the epitope tag in a variety of configurations. pCMV-Tag vectors have a single copy of the available epitope tags, resulting in weaker tagging.  Stronger signal in immunological assays  Small tags do not interfere with functional analysis studies  Three reading frames for both N- and C- terminus tags

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