RNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.
The Affinity protein expression and purification system provides a simple, gentle and effective method for high-level protein expression and purification. The complete system includes pCAL expression vectors containing a calmodulin-binding peptide tag and calmodulin affinity resin (available separately). pCAL vectors derived from T7 RNA polymerase-based pET vectors€_€_ Novel purification tag combines small size, gentle el…
Dose-responsive inducible mammalian expression Induction as high as 1,700 fold Tightly repressed Novel IRES site allows expression of both receptor subunits from the same CMV promoter Retroviral and plasmid gene delivery systems available Stable receptor cell lines available
Epitope-tagging technology provides you a powerful means to functionally analyze your protein of interest without the need for an antibody specific to each new protein under study.
The pCMV-3Tag vectors are a series of epitope tagging mammalian expression vectors featuring three copies of the epitope tag in a variety of configurations. pCMV-Tag vectors have a single copy of the available epitope tags, resulting in weaker tagging. Stronger signal in immunological assays Small tags do not interfere with functional analysis studies Three reading frames for both N- and C- terminus tags
The pCMV-3Tag vectors are a series of epitope tagging mammalian expression vectors featuring three copies of the epitope tag in a variety of configurations. pCMV-Tag vectors have a single copy of the available epitope tags, resulting in weaker tagging. Stronger signal in immunological assays Small tags do not interfere with functional analysis studies Three reading frames for both N- and C- terminus tags
The pCMV-3Tag vectors are a series of epitope tagging mammalian expression vectors featuring three copies of the epitope tag in a variety of configurations. pCMV-Tag vectors have a single copy of the available epitope tags, resulting in weaker tagging. Stronger signal in immunological assays Small tags do not interfere with functional analysis studies Three reading frames for both N- and C- terminus tags
The pCMV-3Tag vectors are a series of epitope tagging mammalian expression vectors featuring three copies of the epitope tag in a variety of configurations. pCMV-Tag vectors have a single copy of the available epitope tags, resulting in weaker tagging. Stronger signal in immunological assays Small tags do not interfere with functional analysis studies Three reading frames for both N- and C- terminus tags
The pCMV-3Tag vectors are a series of epitope tagging mammalian expression vectors featuring three copies of the epitope tag in a variety of configurations. pCMV-Tag vectors have a single copy of the available epitope tags, resulting in weaker tagging. Stronger signal in immunological assays Small tags do not interfere with functional analysis studies Three reading frames for both N- and C- terminus tags
The pCMV-3Tag vectors are a series of epitope tagging mammalian expression vectors featuring three copies of the epitope tag in a variety of configurations. pCMV-Tag vectors have a single copy of the available epitope tags, resulting in weaker tagging. Stronger signal in immunological assays Small tags do not interfere with functional analysis studies Three reading frames for both N- and C- terminus tags
The pCMV-3Tag vectors are a series of epitope tagging mammalian expression vectors featuring three copies of the epitope tag in a variety of configurations. pCMV-Tag vectors have a single copy of the available epitope tags, resulting in weaker tagging. Stronger signal in immunological assays Small tags do not interfere with functional analysis studies Three reading frames for both N- and C- terminus tags
The pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1 hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives. The pCMV-Script vector is derived from a high-copy-number pUC-based plasmid and is designed to allow protein expression in mammalian systems. Mammalian expression…
The pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1 hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives.
Epitope-tagging technology provides you a powerful means to functionally analyze your protein of interest without the need for an antibody specific to each new protein under study.
Epitope-tagging technology provides you a powerful means to functionally analyze your protein of interest without the need for an antibody specific to each new protein under study.
Epitope-tagging technology provides you a powerful means to functionally analyze your protein of interest without the need for an antibody specific to each new protein under study.
Epitope-tagging technology provides you a powerful means to functionally analyze your protein of interest without the need for an antibody specific to each new protein under study.
Epitope-tagging technology provides you a powerful means to functionally analyze your protein of interest without the need for an antibody specific to each new protein under study.
The pDual Expression Vector offers high-level heterologous gene expression in both mammalian and prokaryotic systems.
The pDual Expression Vector offers high-level heterologous gene expression in both mammalian and prokaryotic systems.
To express the gene of interest, the pERV3 receptor vector and the pEGSH expression vector are part of the Complete Control Inducible Mammalian Expression System, designed to provide the tightest control of expression available in mammalian cells.
To express the gene of interest,the pERV3 receptor vector and the pEGSH expression vector are part of the Complete Control Inducible Mammalian Expression System, designed to provide the tightest control of expression available in mammalian cells.
The pESC vectors are a series of epitope-tagging vectors for expression of eukaryotic genes in the yeast S. cerevisiae.
The pESC vectors are a series of epitope-tagging vectors for expression of eukaryotic genes in the yeast S. cerevisiae.
The pET E.coli expression system is a widely used in vivo bacterial expression system due to the strong selectivity of the bacteriophage T7 RNA polymerase for its cognate promoter sequences, the high level of activity of the polymerase, and the high efficiency of translation mediated by the T7 gene. Tightly controlled T7 RNA polymerase-based gene expression in E.coli High-level expression upon induction Convenien…
The pET E.coli expression system is a widely used in vivo bacterial expression system due to the strong selectivity of the bacteriophage T7 RNA polymerase for its cognate promoter sequences, the high level of activity of the polymerase, and the high efficiency of translation mediated by the T7 gene. Tightly controlled T7 RNA polymerase-based gene expression in E.coli High-level expression upon induction Convenien…
