RNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.
The Lambda ZAP-CMV vector allows production of libraries in a high-efficiency lambda vector. Libraries cloned into lambda vectors routinely produce greater than 4 times the primary library size as plasmid libraries. The Lambda ZAP II system combines the high efficiency of lambda library construction and the convenience of a plasmid system with improved blue-white color selection. High-efficiency lambda cloning with versatili…
The Lambda ZAP II vectors combine the high efficiency of lambda vector systems with the versatility of plasmid systems. They enable prokaryotic expression of your gene of interest under control of the lac promoter. In vivo excision of the Lambda ZAP II vector yields the pBluescript SK- vector, which is a high-copy number pUC-based phagemid.
The Lambda ZAP II vectors combine the high efficiency of lambda vector systems with the versatility of plasmid systems. They enable prokaryotic expression of your gene of interest under control of the lac promoter. In vivo excision of the Lambda ZAP II vector yields the pBluescript SK- vector, which is a high-copy number pUC-based phagemid.
The pAAV-hrGFP vector is used as a positive control in the AAV Helper-Free System. This control vector uses humanized Renilla GFP (hrGFP) reporter gene for easy detection of expression in vivo using fluorescence microscopy, fluorescence-activated cell sorting (FACS) analysis, or fluorometry.
The pAAV-hrGFP vector is used as a positive control in the AAV Helper-Free System. This control vector uses humanized Renilla GFP (hrGFP) reporter gene for easy detection of expression in vivo using fluorescence microscopy, fluorescence-activated cell sorting (FACS) analysis, or fluorometry.
Recombinant adenoviruses are a versatile tool for gene delivery and expression. Choose pAdEasy-1 vector or any of these three shuttle vectors, pShuttle, pShuttle-CMV, or pShuttle-CMV-lacZ vector, to clone DNA of interest with the AdEasy adenoviral vector system.
The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element. Identify pathway-specific transcription activation Easier,…
The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element. Identify pathway-specific transcription activation Easier,…
The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element. Identify pathway-specific transcription activation Easier,…
The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element. Identify pathway-specific transcription activation Easier,…
