Affinity Purification
The Ceres Nanosciences Nanotrap® Protein particles utilize affinity capture and size exclusion to attract and bind low abundance proteins, while excluding unwanted high abundance and high molecular weight interfering molecules.
The Affinity Removal LC Column - Human Albumin is designed to chromatographically remove the most interfering high-abundance protein (albumin) from human samples (e.g., plasma, serum, urine, or cerebrospinal fluid). They are compatible with most FPLC or HPLC pumps or instruments, including Agilent 1200/1100 instruments.
The Steptavidin is produced by fermentation of S. avidinii in a minimal medium. The protein has a structure with four identical subunits, each containing a binding site for biotin. It is stable over a wide range of pH and decomposes only in the presence of SDS at temperatures above 60 °C. Steptavidin conjugated with an enzyme, i.e. Horseradish Peroxidase or Alkaline Phosphatase or a fluorochrome may be used to visualize biot…
The Strep-tag® / Strep-Tactin® purification system allows the isolation of above 95% pure and functional proteins after one chromatographical step. The Strep-tag system is based on the highly selective and easily controllable interaction between the Strep-tag II peptide and the specially engineered streptavidin called Strep-Tactin. The binding affinity of Strep-tag II to Strep-Tactin is nearly 100 times higher than to Stre…
The recently launched Strep-Tactin® Superflow® high capacity resin offers improved binding capacity but identical high protein purity compared to standard Strep-Tactin® Superflow®. Thus, it is the perfect solution for Strep-tag® protein purification. Both Strep-Tactin® Superflow® resins • combine outstanding flow characteristics with superior mechanical stability • are suitable for increased flow rates and useful for purificat…
Strep-Tactin Superflow combines superior mechanical stability and outstanding flow characteristics with high dynamic binding capacity. It is suitable for increased flow rates and is also useful for the purification of large protein complexes. The Strep-tag® / Strep-Tactin® purification system allows the isolation of above 95% pure and functional proteins after one chromatographical step. The Strep-tag system is based on the…
