Enzymes Products & Reviews

25

Enzymes are macromolecular biological catalysts used across life science, pharmaceutical and clinical research. Various enzymes can be purchased for research applications including ligases, high fidelity polymerases, nuclease, protease, restriction enzymes, tissue dissociation enzymes and enzyme inhibitors. Find the best enzymes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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SAP - Shrimp Alkaline Phosphatase

ArcticZymes

ArcticZymes are the inventors of Shrimp Alkaline Phosphatase (SAP). Including its recombinant version (rSAP), it is the first heat-labile, all-purpose alkaline phosphatase, Unlike other alkaline phosphatases, rSAP can be fully inactivated by a short heat treatment of 15 minutes at 65°C. SAP is the gold-standard DNA modifying enzyme, valued for its convenience and versatility. Available in glycerol-free, lyophilization friendl…

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Cod UNG

ArcticZymes

ArcticZymes are the inventors of Cod Uracil-DNA Glycosylase (Cod UNG). Developed from Atlantic Cod it is the only commercially available UNG enzyme that is completely and irreversibly inactivated by moderate heat treatment. The enzyme is produced in a recombinant E. coli (ung-) strain that contains a modified Cod UNG gene. Ideal for PCR Carry-Over Prevention and other applications.

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Isothermal Low Temperature Range

ArcticZymes

ArcticZymes' IsoPol® isothermal polymerases are engineered for rapid, efficient DNA amplification in point-of-care (POC) settings. With enhanced strand displacement, high processivity, and tolerance to biological matrices, they allow faster diagnostics with minimal sample prep. IsoPol BST+ and IsoPol SD+ excel in challenging environments, supporting popular methods like LAMP for quick, reliable results at optimal temperatures.

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Heat&Run gDNA Removal Kit

ArcticZymes

The Heat&Run® gDNA removal kit removes contaminating gDNA from RNA prior to RT-qPCR. The kit is based on the recombinant heat labile dsDNase, which is irreversibly inactivated at low temperatures (5 minutes at 58ºC or 15 minutes at 55ºC). Reverse Transcription can be performed in the same tube, thereby minimizing pipetting steps and reducing hands-on time.

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Endonucleases DNA-specific

ArcticZymes

Double-Strand Specific dsDNase from ArcticZymes is ideal for removing contaminating DNA from PCR master mixes, preventing false positives in bacterial typing. Recombinantly produced in Pichia pastoris, it cleaves dsDNA with 5000-fold higher specificity than ssDNA. Easily inactivated by moderate heat (65°C), it allows for direct addition of DNA templates post-cleanup, ensuring accurate PCR results.

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AZtaq™ DNA Polymerase

ArcticZymes

AZtaq™ DNA Polymerase, derived from Thermus aquaticus, is a high-quality, thermostable enzyme ideal for PCR applications. It catalyzes the synthesis of complementary DNA using a primed template and has 5’-3’ exonuclease activity but lacks 3’-5’ proofreading. AZtaq is compatible with dUTP, allowing efficient carry-over contamination removal when used with Cod UNG.

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Endonucleases Non-Specific, HL-SAN

ArcticZymes

HL-SAN efficiently removes nucleic acids from buffers in protein purification, excelling in high-salt conditions to reduce aggregation. Ideal for removing nucleic acids from proteins with high DNA/RNA affinity, it performs well during lysis, early purification stages, and high-salt eluates. This cold-adapted enzyme works effectively at ambient temperatures and during overnight digestion at 4°C. Triton free available.

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Bioprocessing Salt Active Nucleases - Physiological Conditions

ArcticZymes

Nonspecific endonuclease is highly active at physiological conditions (125–200 mM) for DNA removal during protein production, vaccine manufacturing, and viral vector preparation. M-SAN High Quality can be used directly in cell media, which improves efficiency and yield. Designed to replace Benzonase without workflow changes. M-SAN HQ excels in ensuring higher purity and efficiency. High purity (≥ 99%). Compatible with ELISA ki…

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Endonucleases DNA-specific, HL-dsDNase

ArcticZymes

HL-dsDNase efficiently removes genomic DNA from RNA with minimal impact on RNA quality, reducing 50 ng of gDNA to undetectable levels by qPCR. Easily inactivated by 5-minute incubation at 58°C, it’s ideal for RNA prep, minimizing degradation. It's also effective for removing external DNA from samples before metagenomic sequencing and STR-profiling, for fast, efficient alternative to traditional methods. Triton free available.

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GoTaq® G2 Hot Start Master Mixes

Promega Corp.

The GoTaq® G2 Hot Start Polymerase and Master Mixes simplify your reaction preparation, bringing you the convenience of room-temperature setup and the specificity of a hot start polymerase.   Ready-to-use Master Mix simplifies reaction setup and allows direct-to-gel loading. The optimized formulation provides amplification of a variety of targets, while eliminating non-specific products, resulting in higher yields and gr…

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IdeS Protease

Promega Corp.

IdeS Protease is an immunoglobulin-degrading enzyme from Streptococcus pyogenes (IdeS). It is an engineered recombinant protease overexpressed in E. coli that cleaves Immunoglobulin G (IgG) with high specificity at a single site below the hinge region, yielding F(ab’)2 and Fc fragments. The protocol for a standard reaction is to add the IdeS Protease to the IgG sample, add 1 unit of IdeS Protease per 1µg of IgG to be digested…

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IdeZ Protease

Promega Corp.

IdeZ Protease is an immunoglobulin-degrading enzyme from Streptococcus equi subspecies zooepidemicus. It is an engineered recombinant protease overexpressed in E. coli. Like IdeS Protease, IdeZ Protease specifically cleaves IgG molecules below the hinge region to yield F(ab′)2 and Fc fragments. However, IdeZ Protease has significantly improved activity against mouse IgG2a and IgG3 subclasses compared to IdeS Protease.

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