Protein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
The ready-to-use QuickPick IMAC metal affinity kit is intended for the purification of histidine-tagged proteins using Immobilized Metal Affinity Chromatography. It is optimized for use with the PickPen magnetic particle tool.
The ready-to-use QuickPick IMAC metal affinity kit is intended for the purification of histidine-tagged proteins using Immobilized Metal Affinity Chromatography. It is optimized for use with the PickPen magnetic particle tool.
For use with PickPen magnetic tools when not working with QuickPick products, PickPen standard tips withstand autoclaving to ensure DNAase/RNAase free conditions. To avoid sample cross-contamination, new tips should be taken for each sample. Note that Bio-Nobile's QuickPick kits include all tips needed for the number of preparations specified for the kit.
For use with PickPen magnetic tools when not working with QuickPick products, DNAase/RNAase free PickPen tips are supplied in a convenient plastic pack. Note that Bio-Nobile's QuickPick kits include all tips needed for the number of preparations specified for the kit.
For convenient handling and storage of PickPen tips, the PickPen tip box will hold 96 tips in 8 x 12 configuration in a vertical position allowing them to be picked up easily with PickPen magnetic tools.
Protein A from Staphylococcus aureus has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein A on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.
The Magna GrIP™ Rack is a polyethylene rack containing 4 neodinium magnets. The rack is designed to rapidly and easily pellet samples bound to magnetic particles from the supernatant. In its horizontal position, the rack contains 8 holes suitable for 1.5-2.0 ml tubes or spin columns. The rack is versatile, and may also be used with either 15 ml or alternately, 0.5 ml tubes, simply by standing the rack on either side.
Streptavidin isolated from Streptomyces avidinii covalently bound to agarose.
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP's, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release® is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been…
Montage Antibody Purification Kits are designed for fast and easy antibody purification from serum, ascites or cell culture supernatants. The kits include spin columns pre-packed with high capacity PROSEP-A or G media. There are no pumps to setup. No columns to pack. All you need is a centrifuge to purify 10–20 mg in less than 60 minutes.The protocol is easy to follow and, unlike traditional gravity flow methods, the use of ce…
Automation compatible filter units from EMD Millipore are specially designed to provide efficient filtration for dissolution and other automated sample preparation applications.Choice of Filter Media: Automation compatible filter units are available in a variety of membrane types and pore sizes, including units with prefilters for particulate-laden samples.Automation Compatible Packaging: The 200 pack comes preassembled in 8 t…
Millex filter units with hydrophobic Fluoropore membrane are ideal for sterilizing gases, venting sterile containers, and sterilizing or clarifying organic solutions. Specialized filter units protect hemodialysis transducers from blood and moisture. 50 mm Millex filter units are also used for vacuum line protection. All units are bi-directional
This 13 mm syringe filter with a Hydrophilic Polyethersulfone membrane, for non-sterile filtration, has a 0.45 micron pore size used in preparation of aqueous & mild organic solutions prior to chromatography or other instrument analyses.
Millex-GP Filter for Samplicity G2, 0.22 µm, PES 33 mm, non-sterile
The PickPen 8-M magnetic tool offers the same benefits as the PickPen 1-M but allows parallel processing of 8 samples. The device is suitable for use with QuickPick™ kits in microplate applications.
The magnetic bead separation device Magnetic PlateX can be used with a Xiril pipetting workstation for automated nucleic acid purification based on the magnetic beads technology. Speed up the process of nucleic acid purification with a magnetic beads kit using the Magnetic PlateX on a Xiril robotic workstation. All pipetting steps are taken over by the robot and the plate gripper “Plate GripX” automatically carries out the tra…
It can be challenging to purify sufficient high-quality protein from mammalian cells. Boost protein recovery 30-60% with an optimized covalent protein tag, called HaloTag®. This complete kit offers strong improvements in recovery, purity, cost and protein functionality over historical FLAG® and poly-His tag purification strategies.
Superior Bead Recovery and Low Levels of False Positives Ensure Assay Reproducibility. Smooth well wall provides efficient bead recovery, ensuring reproducible results lot after lot High performance membrane does not trap microspheres in the membrane matrix In serological assays, Supor® membrane effectively removes IgG complexes, thus reducing nonspecific reactivity of the microspheres and reducing false positives New well des…
Robust Chemical Resistance Provides Compatibility with Harsh Organics. Chemically resistant materials provide a stable platform to process samples in organic solvents Inert materials of construction ensure complete recovery of samples and low non-specific binding New well design results in faster, more uniform filtration rates across the plate and reduces hold-up volume Innovative outlet tip geometry provides direct flow of sa…
QuickPick™ GST kit for proteins, 48 preps (62411)The ready-to-use QuickPick GST glutathione affinity kit is intended for the purification of GST (glutathione-S-transferase)-tagged proteins. It is optimized for use with the PickPen 1-M magnetic tool, and at least 40 ug tagged protein can be purified per prep.
BugBuster® Master Mix combines BugBuster Protein Extraction Reagent with Benzonase® Nuclease and rLysozyme™ Solution in one convenient reagent. BugBuster Master Mix allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. With the Master Mix, there is no need for dilution or separate addition steps. The two available package sizes provide sufficient reagents for protein extracti…
