Proteomics Products & Reviews

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Proteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.

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Typhoon laser-scanner platform

Cytiva

Amersham™ brand Typhoon™ laser-scanner platform provides exceptional data quality through extremely sensitive detection, high image resolution, and a very broad linear dynamic range. These versatile imaging systems support multiple imaging modes, including phosphor imaging, red/green/blue (RGB) and long and short wavelengths of near infrared fluorescence (NIR), as well as optical densitometry (OD) of proteins in stained gels.

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PrismA ELISA kit

Cytiva

The PrismA ELISA kit is an enzyme-linked immunosorbent assay designed to measure residual ligand leakage when using MabSelect PrismA ™ chromatography resin and Fibro ™ PrismA chromatography adsorbers. The kit is used during the development and manufacture of monoclonal antibody (mAb) biopharmaceuticals.

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pET Expression Systems

Agilent Technologies

The pET E. coli expression system is a widely used in vivo bacterial expression system due to the strong selectivity of the bacteriophage T7 RNA polymerase for its cognate promoter sequences, the high level of activity of the polymerase, and the high efficiency of translation mediated by the T7 gene.

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pBK-CMV Phagemid Vector

Agilent Technologies

The pBK-CMV vector allows you to express proteins in both E. coli and mammalian model systems and saves you valuable time by not having to subclone into an additional vector.  In mammalian cells, the expression is driven by the CMV promoter and is therefore constitutively expressed.  In E. coli, the lac promoter drives high-level expression in the presence of the inducer IPTG, and allows minimal expression in the absence of i…

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pCMV-Tag Vectors

Agilent Technologies

Epitope-tagging technology provides you a powerful means to functionally analyze your protein of interest without the need for an antibody specific to each new protein under study. The pCMV-Tag vectors have a single copy of the available epitope tags, while the pCMV-3Tag vectors have three copies of the epitope tag (in a variety of configurations) to prove a stronger signal in immmunological assays.

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pSG5 Vector

Agilent Technologies

The pSG5 Vector is a 4.1 Kb eukaryotic expression vector constructed by combining pKCR2 and the Stratagene pBS vector. The high copy number of this plasmid allows for large quantities of double-stranded DNA to be obtained. Expression in vivo is achieved via transient expression in a variety of cell lines (highest level of expression is obtained following transfection of a cell line expressing the T antigen).

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StrataClean Resins

Agilent Technologies

StrataClean resin eliminates the need to perform phenol:chloroform extractions, which are highly toxic and combustible. The resin is a non-toxic, non-combustible slurry of hydroxylated silica particles that is used to clean up DNA or concentrate dilute protein mixtures.

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Vitality hrGFP & hrGFP II Mammalian Expression Vectors

Agilent Technologies

Our Vitality hrGFP (humanized, Renilla reniformis green fluorescent protein) products are optimal for mammalian expression protein studies that examine gene expression, transfection efficiency, and protein localization. Since hrGFP is derived from a different organism than EGFP, the hrGFP antibody does not cross react with the Aequorea victoria GFP variant-derived EGFP.

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Mycoplasma Plus PCR Primer Set

Agilent Technologies

Our Mycoplasma Plus PCR Primer Set detects infection by all forms of Mycoplasma with a robust single-band PCR product (triplet for Acholeplasma). This kit also includes controls to verify PCR amplification and Mycoplasma genomic DNA. The primer design permits higher annealing temperatures, reducing spurious bands from PCR contaminants.

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GeneMorph II Random Mutagenesis Kits

Agilent Technologies

The GeneMorph II Random Mutagenesis Kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis. Other methods or commercially available kits that rely on Taq DNA polymerase for random mutagenesis have shown biases towards mutating A’s and T’s more frequently than G’s and C’s, which undoubtedly skews representation of random mutant libraries.

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pESC Yeast Epitope Tagging Vectors

Agilent Technologies

The pESC vectors are a series of epitope-tagging vectors for expression of eukaryotic genes in the yeast S. cerevisiae.  Each vector contains GAL1 and GAL10 yeast promoters in opposing orientations. With these vectors you can introduce one or two cloned genes into a yeast host strain under the control of an inducible promoter.

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Absolutely RNA 96 Microprep Kit

Agilent Technologies

Agilent’s Absolutely RNA Product portfolio makes RNA purification from tissue and cell samples easy and fast. Absolutely RNA kits include all the reagents you need for DNA-free total RNA, and miRNA isolation with no additional purchase of DNase required. No heating, no long centrifugation, and no repurification is required. Each absolutely RNA purification kit has been optimized for a specific range of sample sizes and elution…

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