Purified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
The kit is designed for the efficient and convenient isolation of PCR products from amplification reactions. Primers, mineral oil, salts, unincorporated nucleotides, and the thermostable DNA polymerase may inhibit subsequent enzymatic reactions (e.g., labeling, sequencing or cloning of the PCR products). In addition, nucleic acids from other modification reactions (e.g., restriction-endonuclease digests, alkaline-phosphatase t…
The High Pure Viral RNA Kit is intended for general laboratory use and is designed to purify viral RNA from serum or plasma. Viral RNA is used for RT-PCR analysis directly after elution in nuclease-free water. Principle As a prerequisite for the reverse transcription polymerase chain reaction (RT-PCR), viral RNA must be isolated from serum or plasma. Virus lysis is accomplished by incubation of the sample in a special Lysis/Bi…
The High Pure Nucleic Acid Kit is intended for general laboratory use and is designed to purify viral nucleic acids from serum, plasma, or whole blood, using sample volumes of up to 200 µl. When using whole blood, total nucleic acids are isolated, including viral nucleic acids. The purified viral nucleic acid is used for PCR and RT-PCR analysis directly after elution in nuclease-free water. PrincipleAs a prerequisite for PCR o…
Use the High Pure FFPE RNA Micro Kit to isolate total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples for direct use in RT-PCR. The isolated RNA is suitable for relative quantification of mRNA in RT-PCR using real-time PCR systems such as the LightCycler® Carousel-Based System and the LightCycler® 480 System. Isolate total RNA from 1 to 10 µm sections of formalin-fixed, paraffin-embedded tissue (e.g., colon, b…
The Genopure Plasmid Midi Kit is used to prepare purified plasmid DNA in medium quantities known as “midi preps”. Using a modified alkaline lysis method, highly purified plasmid DNA from E. coli (free of RNA contamination) is generated. The kit is designed for the isolation of up to 100 µg of plasmid DNA from bacterial culture. Depending on the number of plasmids, use a 5-ml to 30-ml (high-copy number) or 10-ml to 100-ml (low-…
The Fast-Trap Lentivirus Purification and Concentration Kit contains the reagents, filtration devices, and concentration devices necessary to purify the virus away from cellular contaminants and the expressed recombinant transgene. It yields concentrated virus in the exchange buffer of choice, suitable for in vitro and animal studies. The kit provides a quick, easy, membrane-based method for the laboratory scale purification o…
The Fast-Trap Adenovirus Purification and Concentration Kit contains the reagents, filtration devices, and concentration devices necessary to purify the virus away from cellular contaminants and the expressed recombinant transgene. It yields concentrated virus in the exchange buffer of choice, suitable for in vitro and animal studies. The kit provides a quick, easy, membrane-based method for the laboratory scale purification o…
Built on Solid Phase Reversible Immobilization (SPRI) paramagnetic bead-based technology, the Agencourt DNAdvance kit is a high throughput genomic DNA (gDNA) isolation reagent kit enabling the purification of high quality DNA from mammalian tissue samples.
The Empore™ High Performance Extraction Disk Plate differs in design from plates offered by other manufacturers and requires a specific vacuum manifold. Manifolds supplied by different plate manufacturers are specific to the design of their plates and cannot generally be used with plates from other manufacturers. In some cases, the use of spacers to raise/lower the height of a collection device inside the manifold may overco…
Replacement gaskets are available with the vacuum manifold assembly. The gasket can become worn over time and the seal between the plate and the manifold will be affected. This may result in poor vacuum pressure and negatively effect flow rates. The gasket should be checked regularly and replaced when it becomes worn.
