Enzymes Products & Reviews

First generation Pfu polymerases, such as cloned Pfu, PfuTurbo, and PfuUltra, have been improved upon. The improved products, PfuUltra II and Herculase II provide all the benefits of the first generation enzymes plus additional features such as speed to results and larger amplicons.  Cloned Pfu DNA Polymerases  First generation     PfuUltra    DNA Polymerases     PfuTurbo    DNA Polymerases

The engineered PfuUltra II Fusion HS DNA Polymerase provides the worlds most accurate copies and the fastest extension times possible. PfuUltra II Fusion HS DNA Polymerase is PfuUltra hotstart DNA polymerase coupled with a fusion polymerase technology and ArchaeMaxx PCR enhancing factor.  The industry standard for ultra high fidelity PCR providing the most accurate copies of DNA  Dramatically reduced extension times allow f…

SP6 RNA Polymerase is a DNA-dependent RNA polymerase with high sequence specificity for SP6 promoter sequences. SP6 RNA polymerase synthesizes RNA 5' to 3' and incorporates 35S, 32P and 33P ribonucleotides. This enzyme is isolated from an overproducing recombinant E. coli clone.

SureStart Taq DNA polymerase is a hot start Taq DNA polymerase. SureStart Taq can be incorporated into PCR protocols previously optimized with Taq DNA polymerase, with little or no modification of cycling parameters or reaction conditions.

Bacteriophage T3 and T7 RNA polymerases are DNA-dependent RNA polymerases with high sequence specificity for T3 or T7 promoters. 

Bacteriophage T3 and T7 RNA polymerases are DNA-dependent RNA polymerases with high sequence specificity for T3 or T7 promoters. 

Taq2000 DNA Polymerase is a highly purified, recombinant Taq DNA polymerase cloned from the thermophilic eubacteria, Thermus aquaticus.

Taq2000 DNA polymerase is a highly purified, recombinant Taq DNA polymerase cloned from the thermophilic eubacteria, Thermus aquaticus.  Ultrapure cloned     Taq    DNA polymerase  Virtually eliminates background artifacts  Most thermostable     Taq    DNA polymerase

Taq2000 DNA polymerase is a highly purified, recombinant Taq DNA polymerase cloned from the thermophilic eubacteria, Thermus aquaticus.  Ultrapure cloned     Taq    DNA polymerase  Virtually eliminates background artifacts  Most thermostable     Taq    DNA polymerase

The PCR grade deoxynucleotide mix includes 25 mM of each standard dNTP (GATC) for use with any PCR application, including real-time quantitative PCR. These reactions buffers contain the optimal magnesium concentration for efficient PCR.

Discover AccuScript high-fidelity reverse transcriptase (RT). AccuScript delivers 3.7X more accuracy than other commercially available RTs and 6.6X more accuracy than AMV RT. The AccuScript PfuUltra II RT-PCR kit provides a complete system in applications that demand high fidelity, specificity, and throughput.

The Agilent AffinityScript Multiple Temperature Reverse Transcriptase (RT) system is optimized to produce high cDNA yields over the broadest temperature range. During analysis the multi-temp RT device also ensures high affinity to primer-template complexes. cDNA can be synthesized in amounts up to 20 kb.

The high-purity DpnI restriction enzyme is ideal for cloning and Southern blot analysis, and used for site-directed mutagenesis. Stringent quality control is achieved via pBluescript phagemid cloning assay. This assay tests the overall quality of the restriction enzyme used when cloning into the multiple cloning site of the pBluescript II phagemid vector.

Easy-A Hi-Fi Cloning Enzyme & Master Mix combines the higher accuracy of a proofreading enzyme with the high cloning efficiency of Taq. PCR products amplified with the Easy-A can be cloned directly, without performing additional steps typically required when amplifying with proofreading polymerases.

Combines the cloning efficiency of     Taq    DNA polymerase with the accuracy of Pfu DNA polymerase  Proofreading DNA polymerase formulation efficiently adds 3€™-A overhangs to PCR amplicons as efficiently as Taq  Easy-A-amplified PCR products are ready to clone into the TOPO TA Cloning vector and other T/U vectors  Fidelity equivalent to     Pfu    DNA polymerase and 6x more accurate than     Taq    DNA polymerase…

Combines the cloning efficiency of     Taq    DNA polymerase with the accuracy of Pfu DNA polymerase  Proofreading DNA polymerase formulation efficiently adds 3€™-A overhangs to PCR amplicons as efficiently as Taq  Easy-A-amplified PCR products are ready to clone into the TOPO TA Cloning vector and other T/U vectors  Fidelity equivalent to     Pfu    DNA polymerase and 6x more accurate than     Taq    DNA polymerase…

The PicoMaxx high fidelity PCR system reliably produces high PCR product yields on a wide variety of templates up to 10 kb. It also provides superior sensitivity by successfully amplifying samples where starting material is limited.  High sensitivity provides maximum PCR reliability  Consistent, robust amplification up to 10 kb  Superior efficiency and yield with our patented ArchaeMaxx polymerase-enhancing factor  Opti…

Widely used in DNA purification, Proteinase K is a broad-spectrum protease from Tritirachium album that is ideal for preparing chromosomal DNA. It cleaves peptide bonds where carbonyl groups are surrounded by aromatic, hydrophobic, or aliphatic amino acids.

Isopropyl-β-D-thiogalactoside (IPTG) is used in combination with X-gal to detect β-galactosidase activity in blue/white selection assays. When the appropriate vectors are used, blue white screening is an important tool for screening and selecting relevant colonies.

Agilent X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, BCIG) is a premium quality chromogenic substrate of β-Galactosidase. It is used with cloning systems that utilize β-galactosidase activity as an indicator of successful cloning.

The Paq5000 DNA polymerase is a robust Taq substitute that is derived from a Pyrococcus species. This enzyme provides equivalent and often better performance to endpoint Taq DNA polymerase amplification and can be substituted easily into many Taq-based procedures.  Robust, cost-effective alternative to Taq  Equal to better yield with faster cycling conditions  Amplify up to 6 kb genomic DNA targets  Easily substituted…

Taq Extender PCR additive improves the reliability and yield of conventional Taq-based PCR amplifications. Taq Extender additive increases the efficiency of Taq DNA polymerase extension reactions during each cycle of PCR by reducing mismatch pausing, resulting in a greater percentage of completed extension reactions.

Herculase II Fusion DNA Polymerases Pfu Ultra DNA Polymerases & PCR Master Mixes PfuUltra II Hotstart DNA Polymerases & PCR Master MixesHerculase II Fusion DNA PolymerasesThe Herculase II fusion DNA polymerase produces superior yields with extension times as short as 15 sec/kb (see Figure), even with genomic DNA targets up to 12kb. Herculase II fusion DNA polymerase helps overcome PCR challenges with successful amplification o…

Agilent Endoglycosidases (formerly ProZyme) release oligosaccharides from glycoproteins and some other substrates.

Agilent Exoglycosidases (formerly ProZyme) are glycoside hydrolase enzymes that cleave the glycosidic bonds at the terminal residues.