16S rRNA Amplicon Sequencing Offers Enhanced Metagenomic Detection

A commonly used phylogenetic marker in metagenomics is the 16S ribosomal RNA (rRNA) gene. This ubiquitous locus is comprised of highly conserved regions flanking nine hyper-variable regions, referred to as V1-V9. This application note demonstrates the utility of the NEXTflex™ 16S V1-V3 Amplicon-Seq Kit combined with the longer read chemistry of Illumina MiSeq for enabling accurate identification of genera present in highly complex microbial communities across a vast number of samples.