3-Hydroxy-3-Methylglutaric acid, HMG in Urine Analyzed with HPLC

In this application note, a selective, specific, and sensitive HPLC method with LCMS analysis has been developed for the determination of 3-hydroxy-3-methylglutaric acid (a.k.a. Meglutol) in urine samples. The method can be also used in the analysis of plasma samples after precipitation of plasma proteins with acetonitrile. The retention was achieved using a Cogent Diamond Hydride Column.