384 Well Microplate Analysis Using the BacMam Histone H3 [pSer10] Cellular Assay Kit in the Corning® HYPERFlask® Cell Culture Vessel

The high throughput analysis of potential drug candidates in a cell based assay screen can be complicated, but new technologies are currently on the market to aid the assay biologist in developing and screening their drug candidates. Two such technologies include the BacMam Histone H3 [pSer10] Cellular Assay Kit and Corning's High Yield PERformance Flask (HYPERFlask) cell culture vessel. The baculovirus-mediated gene delivery system or BacMam in combination with the LanthaScreen® assay technology can be used for the analysis of specific post-translational modifications of histones. This poster describes a protocol combining both technologies using HEK293 cells as the model.