3D Pluripotent Stem Cells: Simplified Culture and Realistic Differentiation

This white paper illustrates the importance of three-dimensional (3D) cell culture in the production of pluripotent stem cell (PSC)-derived models of embryo development and differentiation. It contains a general overview of embryoid bodies (EBs) and a comparison between the various methods used for their generation. There is also a discussion about using teratoma assays versus EBs to validate pluripotency with newly derived PSC lines. Finally, there is a brief description of recent publications illustrating how 3D culture can be used to maximize differentiation.