A Clinical Research Method for the Analysis of Serum Testosterone and Androstenedione

This application note describes an analytically sensitive and selective clinical research method for the analysis of serum testosterone and androstenedione. Excellent method precision was achieved through the use of analytically sensitive and selective sample preparation, chromatography, and MRM mass spectrometry on the ACQUITY UPLC I-Class UPLC System and Xevo TQD. In addition, method accuracy for the analysis of testosterone has been demonstrated through evaluation of CDC HoSt samples.