A combination of HIER and EIER create an optimal protocol for Desmoglein 3

In the development of a new desmoglein 3 (DSG3) antibody, it was noted that both heat retrieval and enzyme retrieval individually gave borderline results in unmasking the epitopes for this antigen. It was hypothesized that a combined retrieval protocol would give optimal staining results when compared to the individual retrieval methods. The results are illustrated in this experiment.