A Fast and Effective Approach to Analyzing Hormones in Drinking Water Using SPE and LC/MS/MS

This application note illustrates a method for steroid hormone analysis from drinking water using an optimized LC/MS/MS method. Analysis time is reduced from 50 minutes down to about 15 minutes while maintaining excellent linearity (0.99 R2 value or greater for each compound) and low minimum reporting level MRLs. The method presented uses a Gemini® 3 μm NX-C18 HPLC column, which delivers stability at pH 1-12. This improved method should greatly increase lab efficiency and productivity.