A Fully Automated, Bottom-up Approach for MALDI-TOF MS Based Discovery Workflows

This application note demonstrates a fully automated solution for MALDI-TOF MS based peptide mapping experiments. Using an online digestion, desalt, reversed phase separation and fraction collection platform, the average experimental time was decreased from over 18 hours to less than 30 min (reversed phase separation included – acquisition time not included) while substantially improving sequence coverage.