A High-Throughput, Homogeneous, FRET-Based Assay to Detect Bacterial Membrane-Bound Enzyme

The activity of an integral membrane protein (MraY) was measured using a FRET-based assay. The FRET donor is coupled to the translocase substrate while the acceptor is embedded in micelles that also contain the enzyme. The assay is useful for searching inhibitors that represent drug targets. The ratiometric measurement was performed simultaneously in 384-well format using the PHERAstar HTS microplate reader from BMG LABTECH.