A high-throughput, multiplex antibody internalization assay

Many fluorescence based methods are available to assess internalization; however, performing these assays can prove challenging due to complicated workflows, large sample sizes and the low throughput of analytical methods such as traditional flow cytometry or confocal microscopy.

In this poster, Sartorius describe a high-throughput, multiplex, no-wash assay that measures antibody internalization, specificity, and cell health from a single 30 uL assay volume in 96- and 384- well formats, with the iQue^® platform.