A High-Throughput, Radioactivity-Free Assay For Cell-Mediated Cytotoxicity

This application note demonstrates a fast, efficient, radioactivity-free CMC assay with low sample input requirements, enabling miniaturization to 384-well plates. Using the iQue^® Screener and differential labeling of effector and target cells, this method can capture multiple facets of biology—apoptosis markers, signal transduction markers, cell permeability, proliferative capability, and more—in a single well, providing a rich and highly quantitative data set specific to each cell type present. With this approach, researchers and drug discovery teams can quickly screen through compounds and conditions, building a detailed understanding of the molecular events occurring in each well and speeding insight into development of immunotherapeutic approaches.