A Homogeneous Multiplex Immunoassay for RTK Phosphorylation State that is Adaptable to HTS

This application note looks at the VersaMAP™ Magnetic Human RTK Multiplex Kits which are Luminex ® bead-based multiplex immunoassays for the analysis of RTK phosphorylation. These assays can be readily adapted for high-throughput screening with minimal manual intervention from cell treatment to data collection. To demonstrate this, several different cell lines were grown in 96-well plates, treated with various stimulants or inhibitors, and lysed in the same plate.