A Homogenous Bioluminescent System for Measuring GTPase, GAP and GEF Activities

GTPases play a major role in various cellular functions such as cell signaling, cell proliferation, cell differentiation, cytoskeleton modulation and cell motility. Deregulation or mutation of these proteins results in serious pathological conditions. Targeting GTPases and their regulators have been challenging due to lack of convenient assays. This poster demonstrates the use of a homogenous bioluminescent assay (GTPase/GAP/GEF-Glo) system to analyze these proteins in a simple, convenient “add-mix-read” format, overcoming the challenges in analyzing activities of GTPases and their regulatory proteins, GTPase Activating Proteins (GAP) and Guanine Nucleotide Exchange Factors (GEF).