A Modified Method for the Selective Enrichment of Listeria

This application note demonstrates the use of a modified UVM-Listeria Selective Enrichment Broth for the selective enrichment of Listeria according to USDA-FSIS guidelines. The combination of various peptones, extracts, salts, and buffer substances enable very good growth of Listeria. The selectivity is due to the antiproliferative substances nalidixic acid and acriflavine hydrochloride. The two-stage enrichment method has demonstrated its value especially with sample materials (meat and meat products) that are characterized by a high level of accompanying flora.