A Modular Approach for Epitope Discovery and High-Resolution Profiling of Humoral Immune Responses

This application note presents a modular discovery workflow for the detailed mapping of epitopes. In contrast to protein based assays, peptide microarrays and peptide ELISA provide high resolution information about the antibody repertoire down to distinct epitopes. This approach allows monitoring of individual epitope patterns as well as epitope spreading and addresses natural sequence diversity and post translational modifications. The workflow can be used for biomarker discovery and validation, patient stratification and companion diagnostic development.