A New Micro-Volume System for Highly Precise Photometric Determination of Nucleic Acids or Proteins in the Eppendorf BioPhotometer® and Eppendorf BioSpectrometer®

The use of the microliter measurement cell μCuvette, in combination with the BioPhotometer or BioSpectrometer, enables determination of high concentrations of nucleic acids or proteins using minimum volumes. In order to achieve optimum results, the measurement principle will be elaborated in this application note, and use and handling of the μCuvette are described in detail. In addition, comparative measurements with other cuvettes and microvolume cuvettes were performed, with a focus on self absorption, precision and accuracy.