A novel, automated, and highly selective phosphopeptide enrichment for phosphopeptide identification and phosphosite localization

Phosphopeptide enrichment is one of the most challenging sample preparation steps for LC/MS analysis due to the variation in reproducibility and selectivity caused by manual sample preparation. In this application note, Agilent Technologies performs an automated phosphopeptide enrichment with both qualitative and quantitative analysis using the Agilent AssayMAP Bravo platform and an LC/Q‑TOF system was implemented for the HUPO Phosphopeptide Challenge.